Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094077760> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2094077760 endingPage "54" @default.
- W2094077760 startingPage "50" @default.
- W2094077760 abstract "The determination of protein tyrosine phosphatase (PTP) activity using different protein substrates such as modified lysozyme or myelin basic protein is greatly affected by the type of enzyme involved, the condition of the assay, and the presence of effectors. The purpose of this study was to develop a general substrate of wide applicability with which variations in enzymatic activity would be minimized. A nonapeptide ENDYINASL derived from a highly conserved region of the T-cell phosphatase TC.PTP (Cool et al. (1989) Proc. Natl. Acad. Sci. USA 86, 5257-5261) was phosphorylated with a recombinant tyrosine kinase domain of the EGF receptor and purified on a C 18 cartridge. Phosphatase activities of intracellular and receptor-linked PTPs were as high as the highest values obtained with the protein substrates. The intracellular, low-molecular-weight PTPs exhibited Km values between 0.5 and 1.3 μM, whereas the receptor forms CD45 and RPTPα gave values of 14 and 35 μM, respectively. All PTPs displayed similar properties toward the peptide including a low pH optimum and inhibition by vanadate, divalent cations, and heparin. Following immunoprecipitation, 1 ng of TC.PTP could be detected with ENDY(P)INASL compared to 10 ng in presence of protein substrates." @default.
- W2094077760 created "2016-06-24" @default.
- W2094077760 creator A5025231358 @default.
- W2094077760 creator A5038922448 @default.
- W2094077760 creator A5063208606 @default.
- W2094077760 creator A5067706652 @default.
- W2094077760 creator A5070801716 @default.
- W2094077760 creator A5077552332 @default.
- W2094077760 date "1993-05-01" @default.
- W2094077760 modified "2023-10-17" @default.
- W2094077760 title "A General Peptide Substrate for Protein Tyrosine Phosphatases" @default.
- W2094077760 doi "https://doi.org/10.1006/abio.1993.1231" @default.
- W2094077760 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8323038" @default.
- W2094077760 hasPublicationYear "1993" @default.
- W2094077760 type Work @default.
- W2094077760 sameAs 2094077760 @default.
- W2094077760 citedByCount "59" @default.
- W2094077760 countsByYear W20940777602012 @default.
- W2094077760 countsByYear W20940777602013 @default.
- W2094077760 countsByYear W20940777602017 @default.
- W2094077760 countsByYear W20940777602018 @default.
- W2094077760 countsByYear W20940777602022 @default.
- W2094077760 crossrefType "journal-article" @default.
- W2094077760 hasAuthorship W2094077760A5025231358 @default.
- W2094077760 hasAuthorship W2094077760A5038922448 @default.
- W2094077760 hasAuthorship W2094077760A5063208606 @default.
- W2094077760 hasAuthorship W2094077760A5067706652 @default.
- W2094077760 hasAuthorship W2094077760A5070801716 @default.
- W2094077760 hasAuthorship W2094077760A5077552332 @default.
- W2094077760 hasConcept C104317684 @default.
- W2094077760 hasConcept C11960822 @default.
- W2094077760 hasConcept C153911025 @default.
- W2094077760 hasConcept C170493617 @default.
- W2094077760 hasConcept C178666793 @default.
- W2094077760 hasConcept C178790620 @default.
- W2094077760 hasConcept C181199279 @default.
- W2094077760 hasConcept C185592680 @default.
- W2094077760 hasConcept C18903297 @default.
- W2094077760 hasConcept C197462201 @default.
- W2094077760 hasConcept C2776165026 @default.
- W2094077760 hasConcept C2777289219 @default.
- W2094077760 hasConcept C2779281246 @default.
- W2094077760 hasConcept C28406088 @default.
- W2094077760 hasConcept C389152 @default.
- W2094077760 hasConcept C40767141 @default.
- W2094077760 hasConcept C55493867 @default.
- W2094077760 hasConcept C71829478 @default.
- W2094077760 hasConcept C74884574 @default.
- W2094077760 hasConcept C79879829 @default.
- W2094077760 hasConcept C85528070 @default.
- W2094077760 hasConcept C86803240 @default.
- W2094077760 hasConcept C97920573 @default.
- W2094077760 hasConceptScore W2094077760C104317684 @default.
- W2094077760 hasConceptScore W2094077760C11960822 @default.
- W2094077760 hasConceptScore W2094077760C153911025 @default.
- W2094077760 hasConceptScore W2094077760C170493617 @default.
- W2094077760 hasConceptScore W2094077760C178666793 @default.
- W2094077760 hasConceptScore W2094077760C178790620 @default.
- W2094077760 hasConceptScore W2094077760C181199279 @default.
- W2094077760 hasConceptScore W2094077760C185592680 @default.
- W2094077760 hasConceptScore W2094077760C18903297 @default.
- W2094077760 hasConceptScore W2094077760C197462201 @default.
- W2094077760 hasConceptScore W2094077760C2776165026 @default.
- W2094077760 hasConceptScore W2094077760C2777289219 @default.
- W2094077760 hasConceptScore W2094077760C2779281246 @default.
- W2094077760 hasConceptScore W2094077760C28406088 @default.
- W2094077760 hasConceptScore W2094077760C389152 @default.
- W2094077760 hasConceptScore W2094077760C40767141 @default.
- W2094077760 hasConceptScore W2094077760C55493867 @default.
- W2094077760 hasConceptScore W2094077760C71829478 @default.
- W2094077760 hasConceptScore W2094077760C74884574 @default.
- W2094077760 hasConceptScore W2094077760C79879829 @default.
- W2094077760 hasConceptScore W2094077760C85528070 @default.
- W2094077760 hasConceptScore W2094077760C86803240 @default.
- W2094077760 hasConceptScore W2094077760C97920573 @default.
- W2094077760 hasIssue "1" @default.
- W2094077760 hasLocation W20940777601 @default.
- W2094077760 hasLocation W20940777602 @default.
- W2094077760 hasOpenAccess W2094077760 @default.
- W2094077760 hasPrimaryLocation W20940777601 @default.
- W2094077760 hasRelatedWork W1966256275 @default.
- W2094077760 hasRelatedWork W1975017809 @default.
- W2094077760 hasRelatedWork W1986604108 @default.
- W2094077760 hasRelatedWork W2076626041 @default.
- W2094077760 hasRelatedWork W2077584190 @default.
- W2094077760 hasRelatedWork W2094077760 @default.
- W2094077760 hasRelatedWork W2131922759 @default.
- W2094077760 hasRelatedWork W2419015479 @default.
- W2094077760 hasRelatedWork W2594670187 @default.
- W2094077760 hasRelatedWork W2507843907 @default.
- W2094077760 hasVolume "211" @default.
- W2094077760 isParatext "false" @default.
- W2094077760 isRetracted "false" @default.
- W2094077760 magId "2094077760" @default.
- W2094077760 workType "article" @default.