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- W2094114310 abstract "Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the fluorescence quenching of the dye 9-amino-6-chloro-2-methoxyacridine, markedly increased upon addition of fusicoccin to the reconstituted system. This effect could not be observed when binding sites and ATPase preparations were separately reconstituted into the proteoliposomes, thus demonstrating that fusicoccin binding to its receptor is a prerequisite for ATPase stimulation." @default.
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- W2094114310 date "1988-11-01" @default.
- W2094114310 modified "2023-09-23" @default.
- W2094114310 title "Functional reconstitution of a proton-translocating system responsive to fusicoccin." @default.
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- W2094114310 doi "https://doi.org/10.1073/pnas.85.21.7849" @default.
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