FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094156099> ?p ?o ?g. }

• W2094156099 endingPage "65" @default.
• W2094156099 startingPage "59" @default.
• W2094156099 abstract "A gene coding for xylanase synthesis in Bacillus subtilis was isolated by direct shotgun cloning using Escherichia coli as a host. Following partial digestion of B. subtilis chromosomal DNA with PstI or EcoRI restriction enzymes, fragments ranging from 3 to 7 kb were introduced into the PstI or EcoRI sites of pBR325. Transformed colonies having lost either the ampicillin or chloramphenicol resistance markers were screened directly on 1 % xylan plates. Out of 8000 transformants, ten xylanase-positive clones were identified by the clearing zone around lysozyme-treated colonies. Further characterization of one of the clones showed that the xylanase gene was present in a 3.9-kb insert within the PstI site of the plasmid pBR325. Retransformation of E. coli strain with the xylanase-positive hybrid plasmid pRH271 showed 100% transformation to xylanase production. The intracellular xylanase produced by the transformed E. coli was purified by ion exchange and gel permeation chromatography. The electrophoretic mobility of the purified xylanase indicated an Mr of 22 000." @default.
• W2094156099 created "2016-06-24" @default.
• W2094156099 creator A5046803421 @default.
• W2094156099 creator A5061117921 @default.
• W2094156099 creator A5062963851 @default.
• W2094156099 date "1983-12-01" @default.
• W2094156099 modified "2023-09-24" @default.
• W2094156099 title "Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli" @default.
• W2094156099 cites W1490864358 @default.
• W2094156099 cites W1568459291 @default.
• W2094156099 cites W1756714181 @default.
• W2094156099 cites W1954130741 @default.
• W2094156099 cites W1991486518 @default.
• W2094156099 cites W1992042701 @default.
• W2094156099 cites W2009674410 @default.
• W2094156099 cites W2014321075 @default.
• W2094156099 cites W2017985046 @default.
• W2094156099 cites W2032776824 @default.
• W2094156099 cites W2050077441 @default.
• W2094156099 cites W2063450941 @default.
• W2094156099 cites W2064276111 @default.
• W2094156099 cites W2074122230 @default.
• W2094156099 cites W2094082118 @default.
• W2094156099 cites W2094859605 @default.
• W2094156099 cites W2124224337 @default.
• W2094156099 cites W2141653906 @default.
• W2094156099 doi "https://doi.org/10.1016/0378-1119(83)90036-7" @default.
• W2094156099 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6423449" @default.
• W2094156099 hasPublicationYear "1983" @default.
• W2094156099 type Work @default.
• W2094156099 sameAs 2094156099 @default.
• W2094156099 citedByCount "77" @default.
• W2094156099 countsByYear W20941560992012 @default.
• W2094156099 countsByYear W20941560992013 @default.
• W2094156099 countsByYear W20941560992014 @default.
• W2094156099 countsByYear W20941560992015 @default.
• W2094156099 countsByYear W20941560992018 @default.
• W2094156099 countsByYear W20941560992020 @default.
• W2094156099 countsByYear W20941560992022 @default.
• W2094156099 crossrefType "journal-article" @default.
• W2094156099 hasAuthorship W2094156099A5046803421 @default.
• W2094156099 hasAuthorship W2094156099A5061117921 @default.
• W2094156099 hasAuthorship W2094156099A5062963851 @default.
• W2094156099 hasConcept C104317684 @default.
• W2094156099 hasConcept C128319531 @default.
• W2094156099 hasConcept C130123407 @default.
• W2094156099 hasConcept C150194340 @default.
• W2094156099 hasConcept C153911025 @default.
• W2094156099 hasConcept C181199279 @default.
• W2094156099 hasConcept C19924922 @default.
• W2094156099 hasConcept C22744801 @default.
• W2094156099 hasConcept C2775859485 @default.
• W2094156099 hasConcept C2777272437 @default.
• W2094156099 hasConcept C2780242733 @default.
• W2094156099 hasConcept C2780397633 @default.
• W2094156099 hasConcept C523546767 @default.
• W2094156099 hasConcept C54355233 @default.
• W2094156099 hasConcept C547475151 @default.
• W2094156099 hasConcept C55493867 @default.
• W2094156099 hasConcept C86803240 @default.
• W2094156099 hasConcept C89423630 @default.
• W2094156099 hasConceptScore W2094156099C104317684 @default.
• W2094156099 hasConceptScore W2094156099C128319531 @default.
• W2094156099 hasConceptScore W2094156099C130123407 @default.
• W2094156099 hasConceptScore W2094156099C150194340 @default.
• W2094156099 hasConceptScore W2094156099C153911025 @default.
• W2094156099 hasConceptScore W2094156099C181199279 @default.
• W2094156099 hasConceptScore W2094156099C19924922 @default.
• W2094156099 hasConceptScore W2094156099C22744801 @default.
• W2094156099 hasConceptScore W2094156099C2775859485 @default.
• W2094156099 hasConceptScore W2094156099C2777272437 @default.
• W2094156099 hasConceptScore W2094156099C2780242733 @default.
• W2094156099 hasConceptScore W2094156099C2780397633 @default.
• W2094156099 hasConceptScore W2094156099C523546767 @default.
• W2094156099 hasConceptScore W2094156099C54355233 @default.
• W2094156099 hasConceptScore W2094156099C547475151 @default.
• W2094156099 hasConceptScore W2094156099C55493867 @default.
• W2094156099 hasConceptScore W2094156099C86803240 @default.
• W2094156099 hasConceptScore W2094156099C89423630 @default.
• W2094156099 hasIssue "1" @default.
• W2094156099 hasLocation W20941560991 @default.
• W2094156099 hasLocation W20941560992 @default.
• W2094156099 hasLocation W20941560993 @default.
• W2094156099 hasLocation W20941560994 @default.
• W2094156099 hasOpenAccess W2094156099 @default.
• W2094156099 hasPrimaryLocation W20941560991 @default.
• W2094156099 hasRelatedWork W1496454862 @default.
• W2094156099 hasRelatedWork W1556683667 @default.
• W2094156099 hasRelatedWork W1975435595 @default.
• W2094156099 hasRelatedWork W1994044819 @default.
• W2094156099 hasRelatedWork W2032842406 @default.
• W2094156099 hasRelatedWork W2081078312 @default.
• W2094156099 hasRelatedWork W2094700936 @default.
• W2094156099 hasRelatedWork W2328510434 @default.
• W2094156099 hasRelatedWork W347137781 @default.
• W2094156099 hasRelatedWork W4234348956 @default.
• W2094156099 hasVolume "26" @default.
• W2094156099 isParatext "false" @default.

• next