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- W2094160933 abstract "The incorporation of [1-14C]oleic acid, elaidic acid, and stearic acid into lipids was studied in swine hepatic microsomes. The results showed these fatty acids were actively incorporated into phospholipids and neutral lipids. The incorporation of labeled fatty acids into phospholipids was markedly stimulated by the addition of glycerol-3-phosphate or lysophosphatidyl choline to the incubation medium. Considerable amounts of fatty acids were incorporated into triglycerides when glycerol-3-phosphate plus high-speed supernatant fraction (S105) was added to the incubation medium, indicating the microsomal fraction requires the S105 for optimum synthesis of triglycerides from glycerol-3-phosphate and fatty acids in swine liver microsomes. As compared to elaidic and stearic acid, however, more oleic acid was incorporated into microsomal phospholipids when glycerol-3-phosphate or lysophosphatidyl choline was added to the incubation medium. The esterification of oleic acid into the triglyceride fraction was higher than those of elaidic and stearic acids in the presence of glycerol-3-phosphate plus S105. All three fatty acids were incorporated predominantly into the phosphatidyl-choline fraction. As compared to elaidic acid and stearic acid, more oleic acid was recovered in the phosphatidyl-choline fraction. A relatively high percentage of total activity of elaidic acid was found in the phosphatidyl-ethanolamine fraction. When lysophosphatidyl choline was used as the fatty-acid acceptor, most of the labeled fatty acids were recovered in the phosphatidyl-choline fraction." @default.
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- W2094160933 date "1982-08-01" @default.
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- W2094160933 title "Incorporation of fatty acids into swine liver microsomes" @default.
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- W2094160933 doi "https://doi.org/10.1016/0006-2944(82)90056-4" @default.
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