FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094162634> ?p ?o ?g. }

• W2094162634 endingPage "1493" @default.
• W2094162634 startingPage "1481" @default.
• W2094162634 abstract "The synthesis of nitric oxide (NO) is limited by the intracellular availability of L-arginine. Here we show that stimulation of NMDA receptors promotes an increase of intracellular L-arginine which supports an increase in the production of NO. Although L-[3H]arginine uptake measured in cultured chick retina cells incubated in the presence of cycloheximide (CHX, a protein synthesis inhibitor) was inhibited approximately 75% at equilibrium, quantitative thin-layer chromatography analysis showed that free intracellular L-[3H]arginine was six times higher in CHX-treated than in control cultures. Extracellular L-[3H]citrulline levels increased threefold in CHX-treated groups, an effect blocked by NG-nitro-L-arginine, a NO synthase (NOS) inhibitor. NMDA promoted a 40% increase of free intracellular L-[3H]arginine in control cultures, an effect blocked by the NMDA antagonist 2-amino 5-phosphonovaleric acid. In parallel, NMDA promoted a reduction of 40-50% in the incorporation of 35[S]methionine or L-[3H]arginine into proteins. Western blot analysis revealed that NMDA stimulates the phosphorylation of eukaryotic elongation factor 2 (eEF2, a factor involved in protein translation), an effect inhibited by (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK801). In conclusion, we have shown that the stimulation of NMDA receptors promotes an inhibition of protein synthesis and a consequent increase of an intracellular L-arginine pool available for the synthesis of NO. This effect seems to be mediated by activation of eEF2 kinase, a calcium/calmodulin-dependent enzyme which specifically phosphorylates and blocks eEF2. The results raise the possibility that NMDA receptor activation stimulates two different calmodulin-dependent enzymes (eEF2 kinase and NOS) reinforcing local NO production by increasing precursor availability together with NOS catalytic activity." @default.
• W2094162634 created "2016-06-24" @default.
• W2094162634 creator A5009358259 @default.
• W2094162634 creator A5046272512 @default.
• W2094162634 creator A5058786567 @default.
• W2094162634 creator A5061400147 @default.
• W2094162634 date "2006-04-21" @default.
• W2094162634 modified "2023-10-16" @default.
• W2094162634 title "Inhibition of protein synthesis by activation of NMDA receptors in cultured retinal cells: a new mechanism for the regulation of nitric oxide production" @default.
• W2094162634 cites W1499618890 @default.
• W2094162634 cites W1635837931 @default.
• W2094162634 cites W1848589202 @default.
• W2094162634 cites W1864472295 @default.
• W2094162634 cites W193203546 @default.
• W2094162634 cites W1968926496 @default.
• W2094162634 cites W1971633875 @default.
• W2094162634 cites W1977204431 @default.
• W2094162634 cites W1980069682 @default.
• W2094162634 cites W1985877433 @default.
• W2094162634 cites W1989927249 @default.
• W2094162634 cites W1990341941 @default.
• W2094162634 cites W1992270666 @default.
• W2094162634 cites W1994044284 @default.
• W2094162634 cites W1996350615 @default.
• W2094162634 cites W1997899894 @default.
• W2094162634 cites W2001353008 @default.
• W2094162634 cites W2002103559 @default.
• W2094162634 cites W2009537865 @default.
• W2094162634 cites W2014722170 @default.
• W2094162634 cites W2015036130 @default.
• W2094162634 cites W2018490518 @default.
• W2094162634 cites W2025348343 @default.
• W2094162634 cites W2037896376 @default.
• W2094162634 cites W2042695353 @default.
• W2094162634 cites W2043345928 @default.
• W2094162634 cites W2044455826 @default.
• W2094162634 cites W2049527269 @default.
• W2094162634 cites W2052301002 @default.
• W2094162634 cites W2058281248 @default.
• W2094162634 cites W2060635494 @default.
• W2094162634 cites W2064996984 @default.
• W2094162634 cites W2069300266 @default.
• W2094162634 cites W2076135374 @default.
• W2094162634 cites W2078047436 @default.
• W2094162634 cites W2080341245 @default.
• W2094162634 cites W2094038408 @default.
• W2094162634 cites W2096473946 @default.
• W2094162634 cites W2101814309 @default.
• W2094162634 cites W2105482032 @default.
• W2094162634 cites W2109851824 @default.
• W2094162634 cites W2110898773 @default.
• W2094162634 cites W2113747326 @default.
• W2094162634 cites W2120404035 @default.
• W2094162634 cites W2133083344 @default.
• W2094162634 cites W2136262964 @default.
• W2094162634 cites W2137433892 @default.
• W2094162634 cites W2142862020 @default.
• W2094162634 cites W2145806440 @default.
• W2094162634 cites W2150443013 @default.
• W2094162634 cites W2169280213 @default.
• W2094162634 cites W2436956706 @default.
• W2094162634 cites W4300580226 @default.
• W2094162634 doi "https://doi.org/10.1111/j.1471-4159.2006.03843.x" @default.
• W2094162634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16606372" @default.
• W2094162634 hasPublicationYear "2006" @default.
• W2094162634 type Work @default.
• W2094162634 sameAs 2094162634 @default.
• W2094162634 citedByCount "28" @default.
• W2094162634 countsByYear W20941626342012 @default.
• W2094162634 countsByYear W20941626342013 @default.
• W2094162634 countsByYear W20941626342014 @default.
• W2094162634 countsByYear W20941626342017 @default.
• W2094162634 countsByYear W20941626342018 @default.
• W2094162634 countsByYear W20941626342019 @default.
• W2094162634 countsByYear W20941626342020 @default.
• W2094162634 countsByYear W20941626342021 @default.
• W2094162634 countsByYear W20941626342022 @default.
• W2094162634 crossrefType "journal-article" @default.
• W2094162634 hasAuthorship W2094162634A5009358259 @default.
• W2094162634 hasAuthorship W2094162634A5046272512 @default.
• W2094162634 hasAuthorship W2094162634A5058786567 @default.
• W2094162634 hasAuthorship W2094162634A5061400147 @default.
• W2094162634 hasBestOaLocation W20941626341 @default.
• W2094162634 hasConcept C134018914 @default.
• W2094162634 hasConcept C170493617 @default.
• W2094162634 hasConcept C185592680 @default.
• W2094162634 hasConcept C2777468819 @default.
• W2094162634 hasConcept C2778128677 @default.
• W2094162634 hasConcept C28406088 @default.
• W2094162634 hasConcept C3675279 @default.
• W2094162634 hasConcept C515207424 @default.
• W2094162634 hasConcept C519581460 @default.
• W2094162634 hasConcept C55493867 @default.
• W2094162634 hasConcept C67018056 @default.
• W2094162634 hasConcept C79879829 @default.
• W2094162634 hasConcept C86803240 @default.
• W2094162634 hasConceptScore W2094162634C134018914 @default.
• W2094162634 hasConceptScore W2094162634C170493617 @default.

• next