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• W2094172362 endingPage "2878" @default.
• W2094172362 startingPage "2870" @default.
• W2094172362 abstract "GsMTx4 is a 34-residue peptide isolated from the tarantula Grammostola spatulata folded into an inhibitory cysteine knot and it selectively affects gating of some mechanosensitive channels. Here we report the effects of cytoplasmic GsMTx4 on the two bacterial channels, MscS and MscL, in giant Escherichia coli spheroplasts. In excised inside-out patches, GsMTx4 sensitized both channels to tension by increasing the opening rate and decreasing the closing rate. With ascending and descending pressure ramps, GsMTx4 increased the gating hysteresis for MscS, a consequence of slower gating kinetics. Quantitative kinetic analysis of the primary C↔O transition showed that the hysteresis is a result of the decreased closing rate. The gating barrier location relative to the open state energy well was unaffected by GsMTx4. A reconstructed energy profile suggests that the peptide prestresses the resting state of MscS, lowering the net barrier to opening and stabilizes the open conformation by ∼8 kT. In excised patches, both MscL and MscS exhibit reversible adaptation, a process separable from inactivation for MscS. GsMTx4 decreased the rate of reversible adaptation for both channels and the MscS recovery rate from the inactivation. These measurements support a mechanism where GsMTx4 binds to the lipid interface of the channel, increasing the local stress that is sensed by the channels and stabilizing the expanded conformations." @default.
• W2094172362 created "2016-06-24" @default.
• W2094172362 creator A5021397143 @default.
• W2094172362 creator A5045448769 @default.
• W2094172362 creator A5060117994 @default.
• W2094172362 creator A5078991165 @default.
• W2094172362 date "2010-11-01" @default.
• W2094172362 modified "2023-09-29" @default.
• W2094172362 title "Effects of GsMTx4 on Bacterial Mechanosensitive Channels in Inside-Out Patches from Giant Spheroplasts" @default.
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• W2094172362 doi "https://doi.org/10.1016/j.bpj.2010.09.022" @default.
• W2094172362 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2965992" @default.
• W2094172362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21044584" @default.
• W2094172362 hasPublicationYear "2010" @default.
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