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- W2094190226 abstract "Non-native oligomeric forms of biopharmaceutical proteins are therapeutically inactive, and potentially toxic and immunogenic, and therefore undesirable in pharmaceutical formulations. Immunoglobulin G class of antibodies are known to form stable nonnative oligomers through Fab–Fab interactions. In this paper, we investigate thermal-cycling as a technique for disaggregating antibody oligomers. Aggregate containing monoclonal antibody (mAb) samples were exposed to rapid heating and cooling cycles in a thermal-cycler. The heating phase of the thermal-cycle resulted in partial unfolding of the Fab domain, leading to the release of monomer from the oligomer complexes, whereas the rapid cooling that followed led to refolding and minimized the probability of protein reaggregation. The extent of mAb oligomer disaggregation was determined by size-exclusion chromatography and hydrophobic interaction membrane chromatography, whereas protein refolding was assessed by circular dichroism spectroscopy. The thermal-cycling technique in addition to being suitable for disaggregating protein oligomer samples could also potentially be useful for studying the mechanisms of protein aggregation and disaggregation." @default.
- W2094190226 created "2016-06-24" @default.
- W2094190226 creator A5034228225 @default.
- W2094190226 creator A5070530866 @default.
- W2094190226 date "2014-03-01" @default.
- W2094190226 modified "2023-09-23" @default.
- W2094190226 title "A Thermal-Cycling Method for Disaggregating Monoclonal Antibody Oligomers" @default.
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- W2094190226 doi "https://doi.org/10.1002/jps.23863" @default.
- W2094190226 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24549731" @default.
- W2094190226 hasPublicationYear "2014" @default.
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