FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094294562> ?p ?o ?g. }

• W2094294562 endingPage "18227" @default.
• W2094294562 startingPage "18214" @default.
• W2094294562 abstract "Protease serine member S31 (Prss31)/transmembrane tryptase/tryptase-γ is a mast cell (MC)-restricted protease of unknown function that is retained on the outer leaflet of the plasma membrane when MCs are activated. We determined the nucleotide sequences of the Prss31 gene in different mouse strains and then used a Cre/loxP homologous recombination approach to create a novel Prss31−/− C57BL/6 mouse line. The resulting animals exhibited no obvious developmental abnormality, contained normal numbers of granulated MCs in their tissues, and did not compensate for their loss of the membrane tryptase by increasing their expression of other granule proteases. When Prss31-null MCs were activated with a calcium ionophore or by their high affinity IgE receptors, they degranulated in a pattern similar to that of WT MCs. Prss31-null mice had increased baseline airway reactivity to methacholine but markedly reduced experimental chronic obstructive pulmonary disease and colitis, thereby indicating both beneficial and adverse functional roles for the tryptase. In a cigarette smoke-induced model of chronic obstructive pulmonary disease, WT mice had more pulmonary macrophages, higher histopathology scores, and more fibrosis in their small airways than similarly treated Prss31-null mice. In a dextran sodium sulfate-induced acute colitis model, WT mice lost more weight, had higher histopathology scores, and contained more Cxcl-2 and IL-6 mRNA in their colons than similarly treated Prss31-null mice. The accumulated data raise the possibility that inhibitors of this membrane tryptase may provide additional therapeutic benefit in the treatment of humans with these MC-dependent inflammatory diseases. Protease serine member S31 (Prss31)/transmembrane tryptase/tryptase-γ is a mast cell (MC)-restricted protease of unknown function that is retained on the outer leaflet of the plasma membrane when MCs are activated. We determined the nucleotide sequences of the Prss31 gene in different mouse strains and then used a Cre/loxP homologous recombination approach to create a novel Prss31−/− C57BL/6 mouse line. The resulting animals exhibited no obvious developmental abnormality, contained normal numbers of granulated MCs in their tissues, and did not compensate for their loss of the membrane tryptase by increasing their expression of other granule proteases. When Prss31-null MCs were activated with a calcium ionophore or by their high affinity IgE receptors, they degranulated in a pattern similar to that of WT MCs. Prss31-null mice had increased baseline airway reactivity to methacholine but markedly reduced experimental chronic obstructive pulmonary disease and colitis, thereby indicating both beneficial and adverse functional roles for the tryptase. In a cigarette smoke-induced model of chronic obstructive pulmonary disease, WT mice had more pulmonary macrophages, higher histopathology scores, and more fibrosis in their small airways than similarly treated Prss31-null mice. In a dextran sodium sulfate-induced acute colitis model, WT mice lost more weight, had higher histopathology scores, and contained more Cxcl-2 and IL-6 mRNA in their colons than similarly treated Prss31-null mice. The accumulated data raise the possibility that inhibitors of this membrane tryptase may provide additional therapeutic benefit in the treatment of humans with these MC-dependent inflammatory diseases." @default.
• W2094294562 created "2016-06-24" @default.
• W2094294562 creator A5003062246 @default.
• W2094294562 creator A5006764580 @default.
• W2094294562 creator A5007640637 @default.
• W2094294562 creator A5026626784 @default.
• W2094294562 creator A5043300180 @default.
• W2094294562 creator A5050751250 @default.
• W2094294562 creator A5058711635 @default.
• W2094294562 creator A5060451719 @default.
• W2094294562 creator A5066584287 @default.
• W2094294562 creator A5072697342 @default.
• W2094294562 creator A5076048446 @default.
• W2094294562 creator A5078547237 @default.
• W2094294562 creator A5080942972 @default.
• W2094294562 date "2014-06-01" @default.
• W2094294562 modified "2023-10-18" @default.
• W2094294562 title "Importance of Mast Cell Prss31/Transmembrane Tryptase/Tryptase-γ in Lung Function and Experimental Chronic Obstructive Pulmonary Disease and Colitis" @default.
• W2094294562 cites W1483586790 @default.
• W2094294562 cites W1486777323 @default.
• W2094294562 cites W1519269060 @default.
• W2094294562 cites W1521973016 @default.
• W2094294562 cites W1550422318 @default.
• W2094294562 cites W1551914341 @default.
• W2094294562 cites W1565193649 @default.
• W2094294562 cites W1565233457 @default.
• W2094294562 cites W1578242760 @default.
• W2094294562 cites W1777712550 @default.
• W2094294562 cites W1810642313 @default.
• W2094294562 cites W1855226200 @default.
• W2094294562 cites W1903510379 @default.
• W2094294562 cites W1917156299 @default.
• W2094294562 cites W1988705225 @default.
• W2094294562 cites W1992272298 @default.
• W2094294562 cites W1994529392 @default.
• W2094294562 cites W2028338013 @default.
• W2094294562 cites W2040619505 @default.
• W2094294562 cites W2040961976 @default.
• W2094294562 cites W2055492964 @default.
• W2094294562 cites W2063309155 @default.
• W2094294562 cites W2069501078 @default.
• W2094294562 cites W2082848636 @default.
• W2094294562 cites W2087958821 @default.
• W2094294562 cites W2099969611 @default.
• W2094294562 cites W2102718152 @default.
• W2094294562 cites W2103585613 @default.
• W2094294562 cites W2104615437 @default.
• W2094294562 cites W2105122646 @default.
• W2094294562 cites W2110993458 @default.
• W2094294562 cites W2111885508 @default.
• W2094294562 cites W2118764894 @default.
• W2094294562 cites W2125990206 @default.
• W2094294562 cites W2137665821 @default.
• W2094294562 cites W2137748371 @default.
• W2094294562 cites W2140731876 @default.
• W2094294562 cites W2144908001 @default.
• W2094294562 cites W2151851977 @default.
• W2094294562 cites W2154419859 @default.
• W2094294562 cites W2155395286 @default.
• W2094294562 cites W2157425544 @default.
• W2094294562 cites W2158507525 @default.
• W2094294562 cites W2162149799 @default.
• W2094294562 cites W2162384753 @default.
• W2094294562 cites W2398205484 @default.
• W2094294562 cites W349441040 @default.
• W2094294562 cites W4239644485 @default.
• W2094294562 doi "https://doi.org/10.1074/jbc.m114.548594" @default.
• W2094294562 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4140286" @default.
• W2094294562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24821729" @default.
• W2094294562 hasPublicationYear "2014" @default.
• W2094294562 type Work @default.
• W2094294562 sameAs 2094294562 @default.
• W2094294562 citedByCount "78" @default.
• W2094294562 countsByYear W20942945622014 @default.
• W2094294562 countsByYear W20942945622015 @default.
• W2094294562 countsByYear W20942945622016 @default.
• W2094294562 countsByYear W20942945622017 @default.
• W2094294562 countsByYear W20942945622018 @default.
• W2094294562 countsByYear W20942945622019 @default.
• W2094294562 countsByYear W20942945622020 @default.
• W2094294562 countsByYear W20942945622021 @default.
• W2094294562 countsByYear W20942945622022 @default.
• W2094294562 countsByYear W20942945622023 @default.
• W2094294562 crossrefType "journal-article" @default.
• W2094294562 hasAuthorship W2094294562A5003062246 @default.
• W2094294562 hasAuthorship W2094294562A5006764580 @default.
• W2094294562 hasAuthorship W2094294562A5007640637 @default.
• W2094294562 hasAuthorship W2094294562A5026626784 @default.
• W2094294562 hasAuthorship W2094294562A5043300180 @default.
• W2094294562 hasAuthorship W2094294562A5050751250 @default.
• W2094294562 hasAuthorship W2094294562A5058711635 @default.
• W2094294562 hasAuthorship W2094294562A5060451719 @default.
• W2094294562 hasAuthorship W2094294562A5066584287 @default.
• W2094294562 hasAuthorship W2094294562A5072697342 @default.
• W2094294562 hasAuthorship W2094294562A5076048446 @default.
• W2094294562 hasAuthorship W2094294562A5078547237 @default.
• W2094294562 hasAuthorship W2094294562A5080942972 @default.
• W2094294562 hasBestOaLocation W20942945621 @default.

• next