Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094297987> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2094297987 endingPage "1163" @default.
- W2094297987 startingPage "1153" @default.
- W2094297987 abstract "Scleraxis is a transcription factor expressed during early periods of mouse tendon morphogenesis. We have determined that tendon is first clearly present in mouse limb at embryonic day 14.5 (E14.5) and, by in situ hybridization, that scleraxis is expressed in the mouse tendons at E14.5. We have also investigated the regulatory elements that direct scleraxis gene expression to the limb tendons. DNA constructs were engineered such that the lacZ reporter gene was expressed under the control of portions of scleraxis regulatory regions. Transgenic mice carrying these constructs were made and expression of the construct was monitored by staining for beta-galactosidase activity. A construct containing 7 Kbp of 5' flanking sequence, the intron, both exons and 1.8 Kbp of 3' flanking sequence was expressed in a pattern that closely resembled the endogenous scleraxis gene. Mouse embryos carrying this construct expressed lacZ in their limb flexor and extensor tendons at E14.5. The lacZ stain in tendon was readily distinguished from -muscle using an anti-myosin heavy chain antibody to visualize muscle. Deletion of the intron, exons and 3' flanking region did not affect the pattern of tendon expression in the limbs of E14.5 transgenic mice. Additional constructs which deleted 5' flanking sequences up to -355 bp from the published cDNA sequence, showed limb tendon expression that was similar to the endogenous gene. When an additional 160 bp were deleted so that only approximately 200 bp of 5' flanking region was directing lacZ expression, no beta-galactosidase activity was observed in the tendons." @default.
- W2094297987 created "2016-06-24" @default.
- W2094297987 creator A5045320456 @default.
- W2094297987 creator A5061511807 @default.
- W2094297987 creator A5078930789 @default.
- W2094297987 creator A5080260807 @default.
- W2094297987 date "2003-10-01" @default.
- W2094297987 modified "2023-10-16" @default.
- W2094297987 title "Scleraxis (Scx) directs lacZ expression in tendon of transgenic mice" @default.
- W2094297987 cites W1966387132 @default.
- W2094297987 cites W1974078926 @default.
- W2094297987 cites W1992626881 @default.
- W2094297987 cites W1997245179 @default.
- W2094297987 cites W2003708679 @default.
- W2094297987 cites W2019474452 @default.
- W2094297987 cites W2024316133 @default.
- W2094297987 cites W2025105911 @default.
- W2094297987 cites W2034254637 @default.
- W2094297987 cites W2035740045 @default.
- W2094297987 cites W2041440238 @default.
- W2094297987 cites W2048249977 @default.
- W2094297987 cites W2064366726 @default.
- W2094297987 cites W2064879068 @default.
- W2094297987 cites W2081544530 @default.
- W2094297987 cites W2097497927 @default.
- W2094297987 cites W2099281533 @default.
- W2094297987 cites W2106384420 @default.
- W2094297987 cites W2106468261 @default.
- W2094297987 cites W2109194190 @default.
- W2094297987 cites W2113533023 @default.
- W2094297987 cites W2128051624 @default.
- W2094297987 cites W2135466817 @default.
- W2094297987 cites W2163591231 @default.
- W2094297987 doi "https://doi.org/10.1016/j.mod.2003.08.003" @default.
- W2094297987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14568104" @default.
- W2094297987 hasPublicationYear "2003" @default.
- W2094297987 type Work @default.
- W2094297987 sameAs 2094297987 @default.
- W2094297987 citedByCount "25" @default.
- W2094297987 countsByYear W20942979872013 @default.
- W2094297987 countsByYear W20942979872015 @default.
- W2094297987 countsByYear W20942979872016 @default.
- W2094297987 countsByYear W20942979872017 @default.
- W2094297987 countsByYear W20942979872018 @default.
- W2094297987 countsByYear W20942979872020 @default.
- W2094297987 countsByYear W20942979872021 @default.
- W2094297987 countsByYear W20942979872022 @default.
- W2094297987 crossrefType "journal-article" @default.
- W2094297987 hasAuthorship W2094297987A5045320456 @default.
- W2094297987 hasAuthorship W2094297987A5061511807 @default.
- W2094297987 hasAuthorship W2094297987A5078930789 @default.
- W2094297987 hasAuthorship W2094297987A5080260807 @default.
- W2094297987 hasBestOaLocation W20942979871 @default.
- W2094297987 hasConcept C102230213 @default.
- W2094297987 hasConcept C104317684 @default.
- W2094297987 hasConcept C141035611 @default.
- W2094297987 hasConcept C150194340 @default.
- W2094297987 hasConcept C153911025 @default.
- W2094297987 hasConcept C161733203 @default.
- W2094297987 hasConcept C170344550 @default.
- W2094297987 hasConcept C21592294 @default.
- W2094297987 hasConcept C36823959 @default.
- W2094297987 hasConcept C54355233 @default.
- W2094297987 hasConcept C86803240 @default.
- W2094297987 hasConcept C94671646 @default.
- W2094297987 hasConceptScore W2094297987C102230213 @default.
- W2094297987 hasConceptScore W2094297987C104317684 @default.
- W2094297987 hasConceptScore W2094297987C141035611 @default.
- W2094297987 hasConceptScore W2094297987C150194340 @default.
- W2094297987 hasConceptScore W2094297987C153911025 @default.
- W2094297987 hasConceptScore W2094297987C161733203 @default.
- W2094297987 hasConceptScore W2094297987C170344550 @default.
- W2094297987 hasConceptScore W2094297987C21592294 @default.
- W2094297987 hasConceptScore W2094297987C36823959 @default.
- W2094297987 hasConceptScore W2094297987C54355233 @default.
- W2094297987 hasConceptScore W2094297987C86803240 @default.
- W2094297987 hasConceptScore W2094297987C94671646 @default.
- W2094297987 hasIssue "10" @default.
- W2094297987 hasLocation W20942979871 @default.
- W2094297987 hasLocation W20942979872 @default.
- W2094297987 hasOpenAccess W2094297987 @default.
- W2094297987 hasPrimaryLocation W20942979871 @default.
- W2094297987 hasRelatedWork W1988660750 @default.
- W2094297987 hasRelatedWork W2005416777 @default.
- W2094297987 hasRelatedWork W2029733737 @default.
- W2094297987 hasRelatedWork W2037918956 @default.
- W2094297987 hasRelatedWork W2039352533 @default.
- W2094297987 hasRelatedWork W2067166178 @default.
- W2094297987 hasRelatedWork W2068009492 @default.
- W2094297987 hasRelatedWork W2092554027 @default.
- W2094297987 hasRelatedWork W2093633615 @default.
- W2094297987 hasRelatedWork W4242521457 @default.
- W2094297987 hasVolume "120" @default.
- W2094297987 isParatext "false" @default.
- W2094297987 isRetracted "false" @default.
- W2094297987 magId "2094297987" @default.
- W2094297987 workType "article" @default.