FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094300275> ?p ?o ?g. }

• W2094300275 endingPage "1228" @default.
• W2094300275 startingPage "1217" @default.
• W2094300275 abstract "To elucidate the molecular mechanisms underlying the immunosuppressive effects of emodin isolated from Rheum palmatum L.Human T cells were isolated from the peripheral venous blood of 10 healthy adult donors. Cell viability was analyzed with MTT assay. AO/EB and Annexin V/PI staining and DNA damage assay were used to detect cell apoptosis. Fluorescence staining was used to detect the levels of ROS, the mitochondrial membrane potential and intracellular Ca(2+). Colorimetry was used to detect the levels of MDA and total SOD and GSH/GSSG ratio. The expression and activity of caspase-3, -4, and -9 were detected with Western blotting and a fluorometric assay. Western blotting was also used to detect the expression of Bcl-2, Bax, cytochrome C, and endoplasmic reticulum (ER) markers.Emodin (1, 10, and 100 μmol/L) inhibited the growth of human T cells and induced apoptosis in dose- and time dependent manners. Emodin triggered ER stress and significantly elevated intracellular free Ca(2+) in human T cells. It also disrupted mitochondrial membrane potential, and increased cytosolic level of cytochrome C, and the levels of activated cleavage fragments of caspase-3, -4, and -9 in human T cells. Furthermore, emodin significantly increased the levels of ROS and MDA, inhibited both SOD level and GSH/GSSG ratio in human T cells, whereas co-incubation with the ROS scavenger N-acetylcysteine (NAC, 20 μmol/L) almost completely blocked emodin-induced ER stress and mitochondrial dysfunction in human T cells, and decreased the caspase cascade-mediated apoptosis.Emodin exerts immunosuppressive actions at least partly by inducing apoptosis of human T cells, which is triggered by ROS-mediated ER stress and mitochondrial dysfunction." @default.
• W2094300275 created "2016-06-24" @default.
• W2094300275 creator A5004226907 @default.
• W2094300275 creator A5017027072 @default.
• W2094300275 creator A5030352138 @default.
• W2094300275 creator A5033831630 @default.
• W2094300275 creator A5046645216 @default.
• W2094300275 creator A5051764626 @default.
• W2094300275 creator A5055962801 @default.
• W2094300275 creator A5056653649 @default.
• W2094300275 creator A5061597006 @default.
• W2094300275 creator A5081288288 @default.
• W2094300275 date "2013-07-01" @default.
• W2094300275 modified "2023-10-16" @default.
• W2094300275 title "Emodin induces human T cell apoptosis in vitro by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction" @default.
• W2094300275 cites W1604495350 @default.
• W2094300275 cites W1963957869 @default.
• W2094300275 cites W1966223917 @default.
• W2094300275 cites W1971777774 @default.
• W2094300275 cites W1973867263 @default.
• W2094300275 cites W1974114773 @default.
• W2094300275 cites W1992012112 @default.
• W2094300275 cites W2011377721 @default.
• W2094300275 cites W2017554945 @default.
• W2094300275 cites W2019641217 @default.
• W2094300275 cites W2022833626 @default.
• W2094300275 cites W2025511820 @default.
• W2094300275 cites W2044499795 @default.
• W2094300275 cites W2049881247 @default.
• W2094300275 cites W2052252788 @default.
• W2094300275 cites W2057597479 @default.
• W2094300275 cites W2064950130 @default.
• W2094300275 cites W2081522276 @default.
• W2094300275 cites W2081605103 @default.
• W2094300275 cites W2082858693 @default.
• W2094300275 cites W2084299658 @default.
• W2094300275 cites W2105209059 @default.
• W2094300275 cites W2130702747 @default.
• W2094300275 cites W2136729555 @default.
• W2094300275 cites W2137368432 @default.
• W2094300275 cites W2165130076 @default.
• W2094300275 cites W4246546690 @default.
• W2094300275 doi "https://doi.org/10.1038/aps.2013.58" @default.
• W2094300275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4003158" @default.
• W2094300275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23811723" @default.
• W2094300275 hasPublicationYear "2013" @default.
• W2094300275 type Work @default.
• W2094300275 sameAs 2094300275 @default.
• W2094300275 citedByCount "75" @default.
• W2094300275 countsByYear W20943002752013 @default.
• W2094300275 countsByYear W20943002752014 @default.
• W2094300275 countsByYear W20943002752015 @default.
• W2094300275 countsByYear W20943002752016 @default.
• W2094300275 countsByYear W20943002752017 @default.
• W2094300275 countsByYear W20943002752018 @default.
• W2094300275 countsByYear W20943002752019 @default.
• W2094300275 countsByYear W20943002752020 @default.
• W2094300275 countsByYear W20943002752021 @default.
• W2094300275 countsByYear W20943002752022 @default.
• W2094300275 countsByYear W20943002752023 @default.
• W2094300275 crossrefType "journal-article" @default.
• W2094300275 hasAuthorship W2094300275A5004226907 @default.
• W2094300275 hasAuthorship W2094300275A5017027072 @default.
• W2094300275 hasAuthorship W2094300275A5030352138 @default.
• W2094300275 hasAuthorship W2094300275A5033831630 @default.
• W2094300275 hasAuthorship W2094300275A5046645216 @default.
• W2094300275 hasAuthorship W2094300275A5051764626 @default.
• W2094300275 hasAuthorship W2094300275A5055962801 @default.
• W2094300275 hasAuthorship W2094300275A5056653649 @default.
• W2094300275 hasAuthorship W2094300275A5061597006 @default.
• W2094300275 hasAuthorship W2094300275A5081288288 @default.
• W2094300275 hasBestOaLocation W20943002751 @default.
• W2094300275 hasConcept C104317684 @default.
• W2094300275 hasConcept C153911025 @default.
• W2094300275 hasConcept C158617107 @default.
• W2094300275 hasConcept C185592680 @default.
• W2094300275 hasConcept C190283241 @default.
• W2094300275 hasConcept C2776151105 @default.
• W2094300275 hasConcept C2778248515 @default.
• W2094300275 hasConcept C2780783641 @default.
• W2094300275 hasConcept C28859421 @default.
• W2094300275 hasConcept C29311851 @default.
• W2094300275 hasConcept C53227056 @default.
• W2094300275 hasConcept C55493867 @default.
• W2094300275 hasConcept C79879829 @default.
• W2094300275 hasConcept C85265743 @default.
• W2094300275 hasConcept C86803240 @default.
• W2094300275 hasConcept C88634738 @default.
• W2094300275 hasConceptScore W2094300275C104317684 @default.
• W2094300275 hasConceptScore W2094300275C153911025 @default.
• W2094300275 hasConceptScore W2094300275C158617107 @default.
• W2094300275 hasConceptScore W2094300275C185592680 @default.
• W2094300275 hasConceptScore W2094300275C190283241 @default.
• W2094300275 hasConceptScore W2094300275C2776151105 @default.
• W2094300275 hasConceptScore W2094300275C2778248515 @default.
• W2094300275 hasConceptScore W2094300275C2780783641 @default.
• W2094300275 hasConceptScore W2094300275C28859421 @default.
• W2094300275 hasConceptScore W2094300275C29311851 @default.

• next