FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094311039> ?p ?o ?g. }

• W2094311039 endingPage "903" @default.
• W2094311039 startingPage "886" @default.
• W2094311039 abstract "Amide protection factors have been determined from NMR measurements of hydrogen/deuterium amide NH exchange rates measured on assigned signals from Lactobacillus casei apo-DHFR and its binary and ternary complexes with trimethoprim (TMP), folinic acid and coenzymes (NADPH/NADP(+)). The substantial sizes of the residue-specific DeltaH and TDeltaS values for the opening/closing events in NH exchange for most of the measurable residues in apo-DHFR indicate that sub-global or global rather than local exchange mechanisms are usually involved. The amide groups of residues in helices and sheets are those most protected in apo-DHFR and its complexes, and the protection factors are generally related to the tightness of ligand binding. The effects of ligand binding that lead to changes in amide protection are not localised to specific binding sites but are spread throughout the structure via a network of intramolecular interactions. Although the increase in protein stability in the DHFR.TMP.NADPH complex involves increased ordering in the protein structure (requiring TDeltaS energy) this is recovered, to a large extent, by the stronger binding (enthalpic DeltaH) interactions made possible by the reduced motion in the protein. The ligand-induced protection effects in the ternary complexes DHFR.TMP.NADPH (large positive binding co-operativity) and DHFR.folinic acid.NADPH (large negative binding co-operativity) mirror the co-operative effects seen in the ligand binding. For the DHFR.TMP.NADPH complex, the ligand-induced protection factors result in DeltaDeltaG(o) values for many residues being larger than the DeltaDeltaG(o) values in the corresponding binary complexes. In contrast, for DHFR.folinic acid.NADPH, the DeltaDeltaG(o) values are generally smaller than many of those in the corresponding binary complexes. The results indicate that changes in protein conformational flexibility on formation of the ligand complex play an important role in determining the co-operativity in the ligand binding." @default.
• W2094311039 created "2016-06-24" @default.
• W2094311039 creator A5026595457 @default.
• W2094311039 creator A5032365073 @default.
• W2094311039 creator A5037083772 @default.
• W2094311039 date "2006-03-01" @default.
• W2094311039 modified "2023-10-10" @default.
• W2094311039 title "Effects of Co-operative Ligand Binding on Protein Amide NH Hydrogen Exchange" @default.
• W2094311039 cites W1499408335 @default.
• W2094311039 cites W176210329 @default.
• W2094311039 cites W187801720 @default.
• W2094311039 cites W1963584172 @default.
• W2094311039 cites W1964542667 @default.
• W2094311039 cites W1964610252 @default.
• W2094311039 cites W1964877775 @default.
• W2094311039 cites W1977141723 @default.
• W2094311039 cites W1980316342 @default.
• W2094311039 cites W1987343741 @default.
• W2094311039 cites W1990150313 @default.
• W2094311039 cites W2000948557 @default.
• W2094311039 cites W2002380876 @default.
• W2094311039 cites W2004373243 @default.
• W2094311039 cites W2008049884 @default.
• W2094311039 cites W2012451447 @default.
• W2094311039 cites W2015861303 @default.
• W2094311039 cites W2021777305 @default.
• W2094311039 cites W2023305539 @default.
• W2094311039 cites W2024379184 @default.
• W2094311039 cites W2026552146 @default.
• W2094311039 cites W2028231353 @default.
• W2094311039 cites W2028396848 @default.
• W2094311039 cites W2028894926 @default.
• W2094311039 cites W2031094493 @default.
• W2094311039 cites W2042478996 @default.
• W2094311039 cites W2046912614 @default.
• W2094311039 cites W2048424191 @default.
• W2094311039 cites W2051504964 @default.
• W2094311039 cites W2051927163 @default.
• W2094311039 cites W2059150886 @default.
• W2094311039 cites W2060528711 @default.
• W2094311039 cites W2062972508 @default.
• W2094311039 cites W2071659750 @default.
• W2094311039 cites W2071876800 @default.
• W2094311039 cites W2073185240 @default.
• W2094311039 cites W2076366270 @default.
• W2094311039 cites W2080736062 @default.
• W2094311039 cites W2081147529 @default.
• W2094311039 cites W2083143102 @default.
• W2094311039 cites W2087871271 @default.
• W2094311039 cites W2092505006 @default.
• W2094311039 cites W2094605637 @default.
• W2094311039 cites W2094767232 @default.
• W2094311039 cites W2094808269 @default.
• W2094311039 cites W2114320763 @default.
• W2094311039 cites W2114699933 @default.
• W2094311039 cites W2121775643 @default.
• W2094311039 cites W2124649680 @default.
• W2094311039 cites W2128538049 @default.
• W2094311039 cites W2141171773 @default.
• W2094311039 cites W2143705856 @default.
• W2094311039 cites W2169821755 @default.
• W2094311039 cites W2257325031 @default.
• W2094311039 cites W2787731252 @default.
• W2094311039 cites W2951731267 @default.
• W2094311039 cites W976707814 @default.
• W2094311039 doi "https://doi.org/10.1016/j.jmb.2005.11.084" @default.
• W2094311039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16405904" @default.
• W2094311039 hasPublicationYear "2006" @default.
• W2094311039 type Work @default.
• W2094311039 sameAs 2094311039 @default.
• W2094311039 citedByCount "28" @default.
• W2094311039 countsByYear W20943110392012 @default.
• W2094311039 countsByYear W20943110392013 @default.
• W2094311039 countsByYear W20943110392014 @default.
• W2094311039 countsByYear W20943110392015 @default.
• W2094311039 countsByYear W20943110392017 @default.
• W2094311039 countsByYear W20943110392018 @default.
• W2094311039 countsByYear W20943110392020 @default.
• W2094311039 countsByYear W20943110392022 @default.
• W2094311039 crossrefType "journal-article" @default.
• W2094311039 hasAuthorship W2094311039A5026595457 @default.
• W2094311039 hasAuthorship W2094311039A5032365073 @default.
• W2094311039 hasAuthorship W2094311039A5037083772 @default.
• W2094311039 hasConcept C116569031 @default.
• W2094311039 hasConcept C167569220 @default.
• W2094311039 hasConcept C170493617 @default.
• W2094311039 hasConcept C178790620 @default.
• W2094311039 hasConcept C181199279 @default.
• W2094311039 hasConcept C185592680 @default.
• W2094311039 hasConcept C204171352 @default.
• W2094311039 hasConcept C2778439535 @default.
• W2094311039 hasConcept C2781320022 @default.
• W2094311039 hasConcept C47701112 @default.
• W2094311039 hasConcept C512968161 @default.
• W2094311039 hasConcept C55493867 @default.
• W2094311039 hasConcept C71240020 @default.
• W2094311039 hasConcept C8010536 @default.
• W2094311039 hasConcept C86756495 @default.

• next