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• W2094337488 abstract "Mutants of bacteriophage T4D have been isolated whose phenotype differs from that of T4D+ in two respects: (1) the plaque morphology of the mutants is distinguishable from that of T4D+, and (2) the mutants adsorb to bacterial cells at a concentration of polyethylene glycol restrictive for the adsorption of T4D+. These mutants are named CBW mutants. In addition, we received a mutant, T4D.wac, which possesses the phenotype of the CBW mutants. No conditions have been found which are restrictive for the growth of the mutants but permissive for growth of T4D+. The CBW mutants and T4D.wac have been mapped at eight recombinationally distinct sites. These eight loci map into three clusters within which the mutants are genetically linked. One mutant maps in gene 36. Two sites are found in gene 18. The remaining five mutants map at sites lying between the most C-terminal amber mutant mapped in gene 12 and the most N-terminal marker mapped in gene 13. Electron micrographs of the mutants indicate that the five mutants mapping between the terminal markers in genes 12 and 13 are missing the collar and whiskers seen on T4D+ and on the mutants mapping in genes 18 and 36. SDS-polyacrylamide gel electrophoresis of labeled lysates of phage-infected cells shows that these mutants are missing a single, identical protein band, distinct from the protein products of genes 12 and 13. A band of the same molecular weight is missing from gels of purified phage of these five mutants. From this, and the genetic and physiological data, it is suggested that these four CBW mutants and T4D.wac map in a previously undefined gene. It is suggested that this gene is responsible for the presence of collar and whiskers on the phage particle. The gene is called wac (whisker antigen control)." @default.
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• W2094337488 date "1974-03-01" @default.
• W2094337488 modified "2023-10-16" @default.
• W2094337488 title "A new set of adsorption mutants of bacteriophage T4D: Identification of a new gene" @default.
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• W2094337488 doi "https://doi.org/10.1016/0042-6822(74)90153-6" @default.
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