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• W2094367579 abstract "Summary The effect of 1-methyladenine (1-MeA) on adenylate cyclase (AC) basal activity and on preliminary stimulated AC activity was investigated in oocyte membrane preparations of the starfish Aphelasterias japonica. 1-MeA inhibited the membrane-bound AC activity both after its addition to intact oocytes and in cell-free experiments. GTP did not affect AC activity but it intensified the inhibitory effect of 1-MeA on AC activity. Sodium fluoride (F″) stimulated the oocyte AC (8 fold), while 1-MeA significantly reduced F″-stimulated activity. Manganese (MnCl2, 5mM) stimulated AC (150 fold), but 1-MeA did not reduce Mn2+-stimulated activity. However, Mn2+-stimulated AC activity was inhibited by 1-MeA in the presence of MgCl2. Forskolin stimulated AC activity (7 fold) and 1-MeA had no effect on AC. Thus, the inhibitory effect of 1-MeA on stimulated AC activity is displayed only after stimulation of the regulatory AC subunit. We suggest that 1-MeA inhibits the oocyte AC acting via inhibitory regulatory Gi pro..." @default.
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• W2094367579 date "1996-12-01" @default.
• W2094367579 modified "2023-09-25" @default.
• W2094367579 title "1-Methyladenine inhibits adenylate cyclase in starfish oocytes" @default.
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• W2094367579 doi "https://doi.org/10.1080/07924259.1996.9672540" @default.
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