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• W2094389087 abstract "The amino acid binding domains of the tryptophanyl (TrpRS)- and tyrosyl-tRNA synthetases (TyrRS) of Bacillus stearothermophilus are highly homologous. These similarities suggest that conserved residues in TrpRS may be responsible for both determining tryptophan recognition and discrimination against tyrosine. This was investigated by the systematic mutation of TrpRS residues based upon the identity of homologous positions in TyrRS. Of the four residues which interact directly with the aromatic side chain of tryptophan (Phe5, Met129, Asp132, and Val141) replacements of Asp132 led to significant changes in the catalytic efficiency of Trp aminoacylation (200−1250-fold reduction in kcat/KM) and substitution of Val141 by the larger Glu side chain reduced kcat/KM by 300-fold. Mutation of Pro127, which determines the position of active-site residues, did not significantly effect Trp binding. Of the mutants tested, D132N TrpRS also showed a significant reduction in discrimination against Tyr, with Tyr acting as a competitive inhibitor but not a substrate. The analogous residue in B. stearothermophilus TyrRS (Asp176) has also been implicated as a determinant of amino acid specificity in earlier studies [de Prat Gay, G., Duckworth, H. W., and Fersht, A. R. (1993) FEBS Lett. 318, 167−171]. This striking similarity in the function of a highly conserved residue found in both TrpRS and TyrRS provides mechanistic support for a common origin of the two enzymes." @default.
• W2094389087 created "2016-06-24" @default.
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• W2094389087 date "2000-10-01" @default.
• W2094389087 modified "2023-10-15" @default.
• W2094389087 title "Ancient Adaptation of the Active Site of Tryptophanyl-tRNA Synthetase for Tryptophan Binding" @default.
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• W2094389087 doi "https://doi.org/10.1021/bi001512t" @default.
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