Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094405763> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2094405763 endingPage "224" @default.
- W2094405763 startingPage "213" @default.
- W2094405763 abstract "Major intrinsic proteins (MIPs) are believed to contribute towards the maintenance of structural integrity, osmoregulation, and responses to water and salt stresses in higher plants. In this work we identified a 27-kDa MIP (MIP27) in the microsomal membranes from tomato fruit using affinity purified antibodies to MIP27 from Beta vulgaris L. Sucrose gradient centrifugation analysis of microsomal membranes showed that MIP27 was associated with the plasma membrane and tonoplast fractions of tomato fruit. MIP27 aggregated to a 45-kDa protein upon SDS-polyacrylamide gel electrophoresis in the absence of dithiothreitol, a property characteristic of MIP proteins. MIP27 was degraded to a 25-kDa protein as tomato fruit progressed through the various stages of ripeness. The proteolytic degradation of MIP27 to the 25-kDa protein was also observed when microsomal membranes were treated with Pronase E. Treatment of microsomal membranes with thermolysin plus digitonin resulted in the complete degradation of MIP27. MIP27 was insensitive to treatments with trypsin and carboxypeptidase Y. The proteolytic degradation of MIP27 may play a role in the structural integrity and textural properties of tomato fruit during ripening." @default.
- W2094405763 created "2016-06-24" @default.
- W2094405763 creator A5015880535 @default.
- W2094405763 creator A5040902841 @default.
- W2094405763 creator A5049498139 @default.
- W2094405763 date "2000-07-01" @default.
- W2094405763 modified "2023-09-24" @default.
- W2094405763 title "RIPENING-ASSOCIATED PROTEOLYSIS OF A 27-kDa MAJOR INTRINSIC PROTEIN (MBP27) IN TOMATO FRUIT" @default.
- W2094405763 cites W104513617 @default.
- W2094405763 cites W194771144 @default.
- W2094405763 cites W1965893030 @default.
- W2094405763 cites W1968116139 @default.
- W2094405763 cites W1973630093 @default.
- W2094405763 cites W1978354625 @default.
- W2094405763 cites W1982846655 @default.
- W2094405763 cites W1986875713 @default.
- W2094405763 cites W1990225146 @default.
- W2094405763 cites W1994239503 @default.
- W2094405763 cites W2016441295 @default.
- W2094405763 cites W2019408210 @default.
- W2094405763 cites W2040081229 @default.
- W2094405763 cites W2041214205 @default.
- W2094405763 cites W2044480820 @default.
- W2094405763 cites W2054477768 @default.
- W2094405763 cites W2069346782 @default.
- W2094405763 cites W2083898388 @default.
- W2094405763 cites W2090493362 @default.
- W2094405763 cites W2098510298 @default.
- W2094405763 cites W2100837269 @default.
- W2094405763 cites W2105525923 @default.
- W2094405763 cites W2118351852 @default.
- W2094405763 cites W2123822522 @default.
- W2094405763 cites W2128269793 @default.
- W2094405763 cites W2128635872 @default.
- W2094405763 cites W2147285991 @default.
- W2094405763 cites W2164888074 @default.
- W2094405763 cites W4235151569 @default.
- W2094405763 doi "https://doi.org/10.1111/j.1745-4514.2000.tb00697.x" @default.
- W2094405763 hasPublicationYear "2000" @default.
- W2094405763 type Work @default.
- W2094405763 sameAs 2094405763 @default.
- W2094405763 citedByCount "0" @default.
- W2094405763 crossrefType "journal-article" @default.
- W2094405763 hasAuthorship W2094405763A5015880535 @default.
- W2094405763 hasAuthorship W2094405763A5040902841 @default.
- W2094405763 hasAuthorship W2094405763A5049498139 @default.
- W2094405763 hasBestOaLocation W20944057631 @default.
- W2094405763 hasConcept C172353545 @default.
- W2094405763 hasConcept C181199279 @default.
- W2094405763 hasConcept C185592680 @default.
- W2094405763 hasConcept C2780340462 @default.
- W2094405763 hasConcept C2781307694 @default.
- W2094405763 hasConcept C31903555 @default.
- W2094405763 hasConcept C41625074 @default.
- W2094405763 hasConcept C55493867 @default.
- W2094405763 hasConcept C86803240 @default.
- W2094405763 hasConcept C87644729 @default.
- W2094405763 hasConceptScore W2094405763C172353545 @default.
- W2094405763 hasConceptScore W2094405763C181199279 @default.
- W2094405763 hasConceptScore W2094405763C185592680 @default.
- W2094405763 hasConceptScore W2094405763C2780340462 @default.
- W2094405763 hasConceptScore W2094405763C2781307694 @default.
- W2094405763 hasConceptScore W2094405763C31903555 @default.
- W2094405763 hasConceptScore W2094405763C41625074 @default.
- W2094405763 hasConceptScore W2094405763C55493867 @default.
- W2094405763 hasConceptScore W2094405763C86803240 @default.
- W2094405763 hasConceptScore W2094405763C87644729 @default.
- W2094405763 hasIssue "3" @default.
- W2094405763 hasLocation W20944057631 @default.
- W2094405763 hasOpenAccess W2094405763 @default.
- W2094405763 hasPrimaryLocation W20944057631 @default.
- W2094405763 hasRelatedWork W1963486910 @default.
- W2094405763 hasRelatedWork W1984012383 @default.
- W2094405763 hasRelatedWork W2017606803 @default.
- W2094405763 hasRelatedWork W2018936841 @default.
- W2094405763 hasRelatedWork W2019463993 @default.
- W2094405763 hasRelatedWork W2036949783 @default.
- W2094405763 hasRelatedWork W2134817259 @default.
- W2094405763 hasRelatedWork W2317967379 @default.
- W2094405763 hasRelatedWork W2410892619 @default.
- W2094405763 hasRelatedWork W45363377 @default.
- W2094405763 hasVolume "24" @default.
- W2094405763 isParatext "false" @default.
- W2094405763 isRetracted "false" @default.
- W2094405763 magId "2094405763" @default.
- W2094405763 workType "article" @default.