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- W2094413059 abstract "Abstract The interaction of furosemide (FU), one kind of potent diuretic, with bovine serum albumin (BSA) has been investigated at physiological acidity (pH 7.40) by fluorescent technique. Displacement experiment with site markers and Synchronous fluorescence clearly reveal that there are non-specific binding sites of FU with BSA. This conclusion was supported by the binding studies in the presence of the hydrophobic probe 1-anilinonaphthalene-8-sulfonic acid (ANS) and in different ionic strength. The binding sites number n and binding constant K were measured. The thermodynamic parameters Δ H ° , Δ G ° , Δ S ° at different temperatures were calculated. The effects of other four diuretics, some common metal ions and bioactive components from herbal medicine on the binding are also considered. The results show only bumetanide has strong effect on FU’s binding. Moreover, several data processing methods presently used were evaluated with Data of the same set. Quite different results were obtained from these methods suggesting more attention should be paid to the data processing methods." @default.
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- W2094413059 date "2008-01-01" @default.
- W2094413059 modified "2023-10-18" @default.
- W2094413059 title "Characterization of the interaction between furosemide and bovine serum albumin" @default.
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- W2094413059 doi "https://doi.org/10.1016/j.molstruc.2007.02.035" @default.
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