FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094450384> ?p ?o ?g. }

• W2094450384 endingPage "1054" @default.
• W2094450384 startingPage "1050" @default.
• W2094450384 abstract "Wheat RNA ligase can be dissected into three isolated domain enzymes that are responsible for its core ligase, 5'-kinase, and 2',3'-cyclic phosphate 3'-phosphodiesterase activities, respectively. In the present study, we pursued a practical strategy using the domain enzymes for in vitro step-by-step ligation of RNA molecules. As a part of it, we demonstrated that a novel side reaction on 5'-tri/diphosphate RNAs is dependent on ATP, a 2'-phosphate-3'-hydroxyl end, and the ligase domain. Mass spectroscopy and RNA cleavage analyses strongly suggested that it is an adenylylation on the 5' terminus. The ligase domain enzyme showed a high productivity for any of the possible 16 combinations of terminal bases and a high selectivity for the 5'-phosphate and 2'-phosphate-3'-hydroxyl ends. Two RNA molecules having 5'-hydroxyl and 2',3'-cyclic monophosphate groups were ligated almost stoichiometrically after separate conversion of respective terminal phosphate states into reactive ones. As the product has the same terminal state as the starting material, the next rounds of ligation are also possible in principle. Thus, we propose a flexible method for in vitro RNA ligation." @default.
• W2094450384 created "2016-06-24" @default.
• W2094450384 creator A5019414527 @default.
• W2094450384 creator A5022620902 @default.
• W2094450384 creator A5076651757 @default.
• W2094450384 creator A5085701117 @default.
• W2094450384 date "2011-01-01" @default.
• W2094450384 modified "2023-09-27" @default.
• W2094450384 title "Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules" @default.
• W2094450384 cites W110087793 @default.
• W2094450384 cites W1484128229 @default.
• W2094450384 cites W1506905206 @default.
• W2094450384 cites W1518284746 @default.
• W2094450384 cites W1526020850 @default.
• W2094450384 cites W1573106545 @default.
• W2094450384 cites W1588704345 @default.
• W2094450384 cites W1601232054 @default.
• W2094450384 cites W1780093098 @default.
• W2094450384 cites W1965381390 @default.
• W2094450384 cites W1977709085 @default.
• W2094450384 cites W1982534438 @default.
• W2094450384 cites W1991524704 @default.
• W2094450384 cites W1997816658 @default.
• W2094450384 cites W2004601877 @default.
• W2094450384 cites W2006144062 @default.
• W2094450384 cites W2009778542 @default.
• W2094450384 cites W2019442958 @default.
• W2094450384 cites W2020390872 @default.
• W2094450384 cites W2028314046 @default.
• W2094450384 cites W2031615793 @default.
• W2094450384 cites W2037356132 @default.
• W2094450384 cites W2038538652 @default.
• W2094450384 cites W2040584193 @default.
• W2094450384 cites W2100009185 @default.
• W2094450384 cites W2111435786 @default.
• W2094450384 cites W2144450460 @default.
• W2094450384 cites W2164366364 @default.
• W2094450384 cites W2291787412 @default.
• W2094450384 cites W2418469048 @default.
• W2094450384 doi "https://doi.org/10.1016/j.bbrc.2010.12.108" @default.
• W2094450384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21187077" @default.
• W2094450384 hasPublicationYear "2011" @default.
• W2094450384 type Work @default.
• W2094450384 sameAs 2094450384 @default.
• W2094450384 citedByCount "0" @default.
• W2094450384 crossrefType "journal-article" @default.
• W2094450384 hasAuthorship W2094450384A5019414527 @default.
• W2094450384 hasAuthorship W2094450384A5022620902 @default.
• W2094450384 hasAuthorship W2094450384A5076651757 @default.
• W2094450384 hasAuthorship W2094450384A5085701117 @default.
• W2094450384 hasConcept C104317684 @default.
• W2094450384 hasConcept C1342335 @default.
• W2094450384 hasConcept C153911025 @default.
• W2094450384 hasConcept C160403918 @default.
• W2094450384 hasConcept C175114707 @default.
• W2094450384 hasConcept C179437574 @default.
• W2094450384 hasConcept C181199279 @default.
• W2094450384 hasConcept C185592680 @default.
• W2094450384 hasConcept C2777132085 @default.
• W2094450384 hasConcept C2909068217 @default.
• W2094450384 hasConcept C553450214 @default.
• W2094450384 hasConcept C55493867 @default.
• W2094450384 hasConcept C67705224 @default.
• W2094450384 hasConcept C76346623 @default.
• W2094450384 hasConcept C86803240 @default.
• W2094450384 hasConceptScore W2094450384C104317684 @default.
• W2094450384 hasConceptScore W2094450384C1342335 @default.
• W2094450384 hasConceptScore W2094450384C153911025 @default.
• W2094450384 hasConceptScore W2094450384C160403918 @default.
• W2094450384 hasConceptScore W2094450384C175114707 @default.
• W2094450384 hasConceptScore W2094450384C179437574 @default.
• W2094450384 hasConceptScore W2094450384C181199279 @default.
• W2094450384 hasConceptScore W2094450384C185592680 @default.
• W2094450384 hasConceptScore W2094450384C2777132085 @default.
• W2094450384 hasConceptScore W2094450384C2909068217 @default.
• W2094450384 hasConceptScore W2094450384C553450214 @default.
• W2094450384 hasConceptScore W2094450384C55493867 @default.
• W2094450384 hasConceptScore W2094450384C67705224 @default.
• W2094450384 hasConceptScore W2094450384C76346623 @default.
• W2094450384 hasConceptScore W2094450384C86803240 @default.
• W2094450384 hasIssue "4" @default.
• W2094450384 hasLocation W20944503841 @default.
• W2094450384 hasLocation W20944503842 @default.
• W2094450384 hasOpenAccess W2094450384 @default.
• W2094450384 hasPrimaryLocation W20944503841 @default.
• W2094450384 hasRelatedWork W1990486534 @default.
• W2094450384 hasRelatedWork W2001351981 @default.
• W2094450384 hasRelatedWork W2008591193 @default.
• W2094450384 hasRelatedWork W2098211286 @default.
• W2094450384 hasRelatedWork W2160602216 @default.
• W2094450384 hasRelatedWork W2163096453 @default.
• W2094450384 hasRelatedWork W2283065081 @default.
• W2094450384 hasRelatedWork W2914359189 @default.
• W2094450384 hasRelatedWork W35665488 @default.
• W2094450384 hasRelatedWork W4285796434 @default.
• W2094450384 hasVolume "404" @default.
• W2094450384 isParatext "false" @default.
• W2094450384 isRetracted "false" @default.

• next