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• W2094482312 abstract "An enzyme-linked immunosorbent assay (ELISA) using botulinum neurotoxin serotype B recombinant fragment C (rBoNTB(HC)) was developed to measure specific humoral immune responses of monkeys vaccinated with a vaccine consisting of rBoNTB(HC). Several fundamental parameters for a bioassay were evaluated. The evaluation results demonstrated that using BoNTB(HC) as the capture antigen led to a specific and sensitive ELISA for botulinum type B antibody with excellent precision, accuracy, and linearity. There was a good correlation (r=0.91) between ELISA titers and neutralization bioassay titers. Experimental results suggested that the ELISA could be useful for detecting botulinum type B antibody levels and may supplement mouse neutralization bioassays during planned clinical manufacturing and clinical trials of rBoNTB(HC) vaccine." @default.
• W2094482312 created "2016-06-24" @default.
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• W2094482312 date "2003-03-01" @default.
• W2094482312 modified "2023-09-25" @default.
• W2094482312 title "Evaluation of a botulinum fragment C-based ELISA for measuring the humoral immune response in primates" @default.
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• W2094482312 doi "https://doi.org/10.1016/s1045-1056(02)00074-x" @default.
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• W2094482312 hasPublicationYear "2003" @default.
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