Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094482865> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2094482865 endingPage "614" @default.
- W2094482865 startingPage "611" @default.
- W2094482865 abstract "The effect of point mutation in the sequence 316TWLE319, which occurs in the extracellular loop flanking the third (M3) and the fourth (M4) transmembrane segment (L3/4) of the Na+,K+-ATPase alpha-subunit, was examined. Mutation of Glu319 to Asp yielded an enzyme with full activity, whereas substituting Glu319 to Ala resulted in a severe loss of activity. A negative charge was introduced along the sequence, one residue at a time, from Thr316 to Leu318 (by E-scanning) in the mutant construct with Glu319 already mutated to Gln. The activity that had been reduced to 60% by the mutation of Glu319 to Gln was restored upon the introduction of a negative charge by E-scanning. When Leu318 was replaced by Glu in a series of scanning experiments, the K+ sensitivity of the ATPase activity was lowered. The lowering of K+ sensitivity was further demonstrated when a mutation of Leu318 to Glu was introduced into the wild-type enzyme. Furthermore, mutants with Leu318 to Gln, Arg, and Phe displayed lower K+ sensitivity similar to that of Leu318 to Glu mutant. Leu318 may be in access path for K+, and any substitution at this position may interfere with access of K+ from outside the cell." @default.
- W2094482865 created "2016-06-24" @default.
- W2094482865 creator A5022752354 @default.
- W2094482865 creator A5025725057 @default.
- W2094482865 creator A5030131384 @default.
- W2094482865 creator A5057297246 @default.
- W2094482865 creator A5075003293 @default.
- W2094482865 date "2005-05-01" @default.
- W2094482865 modified "2023-10-12" @default.
- W2094482865 title "Involvement in K+ access of Leu318 at the extracellular domain flanking M3 and M4 of the Na+,K+-ATPase α-subunit" @default.
- W2094482865 cites W1495434193 @default.
- W2094482865 cites W1586527248 @default.
- W2094482865 cites W1966112781 @default.
- W2094482865 cites W1968191857 @default.
- W2094482865 cites W1990708284 @default.
- W2094482865 cites W2004071222 @default.
- W2094482865 cites W2009925295 @default.
- W2094482865 cites W2021775911 @default.
- W2094482865 cites W2022703044 @default.
- W2094482865 cites W2051818097 @default.
- W2094482865 cites W2067999317 @default.
- W2094482865 cites W2071210622 @default.
- W2094482865 cites W2074807783 @default.
- W2094482865 cites W2078814797 @default.
- W2094482865 cites W2098550162 @default.
- W2094482865 cites W2110076427 @default.
- W2094482865 cites W2141876893 @default.
- W2094482865 cites W2153331570 @default.
- W2094482865 cites W3025219121 @default.
- W2094482865 cites W4233198827 @default.
- W2094482865 doi "https://doi.org/10.1016/j.bbrc.2005.03.020" @default.
- W2094482865 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15796927" @default.
- W2094482865 hasPublicationYear "2005" @default.
- W2094482865 type Work @default.
- W2094482865 sameAs 2094482865 @default.
- W2094482865 citedByCount "4" @default.
- W2094482865 countsByYear W20944828652015 @default.
- W2094482865 countsByYear W20944828652016 @default.
- W2094482865 crossrefType "journal-article" @default.
- W2094482865 hasAuthorship W2094482865A5022752354 @default.
- W2094482865 hasAuthorship W2094482865A5025725057 @default.
- W2094482865 hasAuthorship W2094482865A5030131384 @default.
- W2094482865 hasAuthorship W2094482865A5057297246 @default.
- W2094482865 hasAuthorship W2094482865A5075003293 @default.
- W2094482865 hasConcept C104292427 @default.
- W2094482865 hasConcept C104317684 @default.
- W2094482865 hasConcept C118892022 @default.
- W2094482865 hasConcept C143065580 @default.
- W2094482865 hasConcept C153911025 @default.
- W2094482865 hasConcept C176944494 @default.
- W2094482865 hasConcept C181199279 @default.
- W2094482865 hasConcept C185592680 @default.
- W2094482865 hasConcept C28406088 @default.
- W2094482865 hasConcept C501734568 @default.
- W2094482865 hasConcept C55493867 @default.
- W2094482865 hasConcept C71240020 @default.
- W2094482865 hasConcept C86803240 @default.
- W2094482865 hasConceptScore W2094482865C104292427 @default.
- W2094482865 hasConceptScore W2094482865C104317684 @default.
- W2094482865 hasConceptScore W2094482865C118892022 @default.
- W2094482865 hasConceptScore W2094482865C143065580 @default.
- W2094482865 hasConceptScore W2094482865C153911025 @default.
- W2094482865 hasConceptScore W2094482865C176944494 @default.
- W2094482865 hasConceptScore W2094482865C181199279 @default.
- W2094482865 hasConceptScore W2094482865C185592680 @default.
- W2094482865 hasConceptScore W2094482865C28406088 @default.
- W2094482865 hasConceptScore W2094482865C501734568 @default.
- W2094482865 hasConceptScore W2094482865C55493867 @default.
- W2094482865 hasConceptScore W2094482865C71240020 @default.
- W2094482865 hasConceptScore W2094482865C86803240 @default.
- W2094482865 hasIssue "2" @default.
- W2094482865 hasLocation W20944828651 @default.
- W2094482865 hasLocation W20944828652 @default.
- W2094482865 hasOpenAccess W2094482865 @default.
- W2094482865 hasPrimaryLocation W20944828651 @default.
- W2094482865 hasRelatedWork W111980633 @default.
- W2094482865 hasRelatedWork W1525684717 @default.
- W2094482865 hasRelatedWork W1550996414 @default.
- W2094482865 hasRelatedWork W1675864211 @default.
- W2094482865 hasRelatedWork W1865915409 @default.
- W2094482865 hasRelatedWork W1965618318 @default.
- W2094482865 hasRelatedWork W1977765199 @default.
- W2094482865 hasRelatedWork W2072906264 @default.
- W2094482865 hasRelatedWork W2073288950 @default.
- W2094482865 hasRelatedWork W2327748221 @default.
- W2094482865 hasVolume "330" @default.
- W2094482865 isParatext "false" @default.
- W2094482865 isRetracted "false" @default.
- W2094482865 magId "2094482865" @default.
- W2094482865 workType "article" @default.