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- W2094508340 abstract "Fluorescence correlation spectroscopy (FCS) is a powerful technique to study dynamic biomolecular processes. It allows the estimation of concentrations, diffusion coefficients, molecular interactions, and other processes causing fluctuations in the fluorescence intensity, thus yielding information about aggregation processes, enzymatic reactions, or partition coefficients. During the last years, FCS has been successfully applied to model and cellular membranes, proving to be a promising tool for the study of membrane dynamics and protein/lipid interactions. Here we describe the theoretical basis of FCS and some practical implications for its application in membrane studies. We discuss sources of potential artifacts, such as membrane undulations, positioning of the detection volume, and photobleaching. Special attention is paid to aspects related to instrumentation and sample preparation as well as data acquisition and analysis. Finally, we comment on some strategies recently developed for the specific improvement of FCS measurements on membranes." @default.
- W2094508340 created "2016-06-24" @default.
- W2094508340 creator A5041672678 @default.
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- W2094508340 date "2008-10-01" @default.
- W2094508340 modified "2023-09-26" @default.
- W2094508340 title "Fluorescence correlation spectroscopy for the study of membrane dynamics and protein/lipid interactions" @default.
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- W2094508340 doi "https://doi.org/10.1016/j.ymeth.2008.06.011" @default.
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