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- W2094535350 abstract "The successful coupling of a relatively small glycoprotein, fetuiu, to ferritin, has been demonstrated by immunoelectrophoresis. By selective destruction of the hemagglutinin with glutaraldehyde followed by interaction with the fetuin-ferritin conjugate, viral neuraminidase could be detected in ultrathin sections of allantoic cells infected with influenza virus. The electron dense label was found surrounding the virions and at the cell surface. The reaction was blocked by treatment of the cells with fetuin, homologous antiviral antibody or brief trypsitization (prior to fixation). Mild acid hydrolysis of the fetuiu, prior to conjugation to ferritin, markedly reduced the binding reaction. It, is suggested that these binding reactions represent a specific enzyme-substrate interaction." @default.
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- W2094535350 date "1967-03-01" @default.
- W2094535350 modified "2023-09-24" @default.
- W2094535350 title "Viral neuraminidase: a cytochemical binding method using a glycoprotein coupled to ferritin" @default.
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- W2094535350 doi "https://doi.org/10.1016/0042-6822(67)90233-4" @default.
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