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• W2094549377 startingPage "8346" @default.
• W2094549377 abstract "We have developed a new highly selective and sensitive technique for the detection of Hg(2+) using DNA-functionalized gold nanoparticles (Au NPs) and OliGreen. This system is the first that allows the detection of Hg(2+) based on the release of DNA molecules, induced by conformational changes on Au NP surfaces, and its sensitivity is highly dependent upon surface DNA density. When Hg(2+) ions interact with the thymidine units of the DNA molecules bound to the Au NPs through Au-S bonds, the conformations of these DNA derivatives change from linear to hairpin structures, causing the release of some of the DNA molecules from the surface of the Au NPs into the bulk solution to react with OliGreen. The fluorescence of OliGreen-DNA complexes increased with increasing concentration of Hg(2+), and Hg(2+) could be detected at concentrations as low as 25 nM. A linear correlation existed between the fluorescence intensity and the concentration of Hg(2+) over the range 0.05-2.5 microM (R(2) = 0.98). This simple and cost-effective probe was applied to determine the spiked Hg(2+) in the pond samples; the recoveries (96-102%) suggested low matrix interference and high sensitivity." @default.
• W2094549377 created "2016-06-24" @default.
• W2094549377 creator A5016268807 @default.
• W2094549377 creator A5035315375 @default.
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• W2094549377 date "2008-06-26" @default.
• W2094549377 modified "2023-10-01" @default.
• W2094549377 title "Control over Surface DNA Density on Gold Nanoparticles Allows Selective and Sensitive Detection of Mercury(II)" @default.
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• W2094549377 doi "https://doi.org/10.1021/la800589m" @default.
• W2094549377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18582003" @default.
• W2094549377 hasPublicationYear "2008" @default.
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