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- W2094578426 abstract "A method for the determination of fructose is described. It is based on the phosphorylation of this sugar by a highly specific fructokinase and the coupling of this reaction to NADP reduction by means of glucosephosphate isomerase and glucose-6-phosphate dehydrogenase. Because of the lack of interference by other sugars, the procedure is appropriate for fructose determination in biological samples where a high content of glucose interferes in other methods currently in use. Even a 100-fold excess glucose causes no detectable interference (less than 1%). The new method has been applied for the estimation of fructose in a series of normal human sera. A mean value of ca. 1 mg per 100 ml was obtained, most serums being within the range 0.5–2." @default.
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- W2094578426 date "1973-07-01" @default.
- W2094578426 modified "2023-10-17" @default.
- W2094578426 title "Fructose determination with a specific fructokinase" @default.
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- W2094578426 doi "https://doi.org/10.1016/0003-2697(73)90264-9" @default.
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