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- W2094636265 abstract "Solid-state nanopore devices with integrated electrodes are an important class of single-molecule biosensors, with potential applications in DNA, RNA, and protein detection and sequence analysis. Here we investigate solid-state nanopore sensors with an embedded gold film, fabricated using semiconductor processing techniques and focused ion beam milling. We characterize their geometric structure in three dimensions on the basis of experimental conductance studies and modeling as well as transmission electron microscopy imaging and tomography. We used electrodeposition to further shrink the pores to effective diameters below 10 nm and demonstrate how bipolar electrochemical coupling across the membrane can lead to significant contributions to the overall pore current and discuss its implications for nanopore sensing. Finally, we use metallized nanopores modified with homocysteine for the detection of insulin. We show that adsorption of the protein to the chemically modified nanopores slows down the translocation process to tens of milliseconds, which is orders of magnitude slower than expected for conventional electrophoretic transport." @default.
- W2094636265 created "2016-06-24" @default.
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- W2094636265 date "2015-01-29" @default.
- W2094636265 modified "2023-10-16" @default.
- W2094636265 title "Electrodeposition and Bipolar Effects in Metallized Nanopores and Their Use in the Detection of Insulin" @default.
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- W2094636265 doi "https://doi.org/10.1021/ac504463r" @default.
- W2094636265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25575083" @default.
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