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- W2094664250 abstract "Sensitive absorption-based detection of anthracycline antibiotics, daunorubicin and doxorubicin is demonstrated using a capillary electrophoresis system interfaced to a nonlinear wave-mixing detection system. Unlike conventional absorption methods, this nonlinear absorption method can detect very thin analytes (50 microm) efficiently. At the same peak height, the wave-mixing CE peak is narrower than a conventional CE peak, and hence, compared to other laser-based or non-laser-based CE on-column detection methods, our wave-mixing detection method offers intrinsically enhanced separation resolution even when using identical CE separation conditions. In this unusually sensitive absorbance detection method, two input laser beams interact to produce a thermally induced grating from which coherent laser-like wave-mixing signal beams are created. Using our sensitive absorbance on-column CE detector, we report a preliminary concentration detection limit of 9.9x10(-10)M using a 50-mum i.d. capillary column. The corresponding injected mass detection limit is 9.1x10(-18)mol using an injection volume of 9.2nL. The corresponding preliminary detected mass detection limit inside the 12-pL detector probe volume is 1.2x10(-20)M." @default.
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- W2094664250 date "2010-10-01" @default.
- W2094664250 modified "2023-10-16" @default.
- W2094664250 title "Sensitive absorption-based wave-mixing detector for anthracycline drugs separated by capillary electrophoresis" @default.
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- W2094664250 doi "https://doi.org/10.1016/j.saa.2010.05.007" @default.
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