Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094667070> ?p ?o ?g. }
- W2094667070 endingPage "34" @default.
- W2094667070 startingPage "18" @default.
- W2094667070 abstract "Staphylococcus aureus is a highly virulent and successful pathogen that causes a diverse array of diseases. Recently, an increase of severe infections in healthy subjects has been observed, caused by community-associated methicillin-resistant S. aureus (CA-MRSA). The reason for enhanced CA-MRSA virulence is unclear; however, work suggests that it results from hypersecretion of agr-regulated toxins, including secreted proteases. In this study, we explore the contribution of exo-proteases to CA-MRSA pathogenesis using a mutant lacking all 10 enzymes. We show that they are required for growth in peptide-rich environments, serum, in the presence of antimicrobial peptides (AMPs), and in human blood. We also reveal that extracellular proteases are important for resisting phagocytosis by human leukocytes. Using murine infection models, we reveal contrasting roles for the proteases in morbidity and mortality. Upon exo-protease deletion, we observed decreases in abscess formation, and impairment during organ invasion. In contrast, we observed hypervirulence of the protease-null strain in the context of mortality. This dichotomy is explained by proteomic analyses, which demonstrates exo-proteases to be key mediators of virulence-determinant stability. Specifically, increased abundance of both secreted (e.g. α-toxin, Psms, LukAB, LukE, PVL, Sbi, γ-hemolysin) and surface-associated (e.g. ClfA+B, FnbA+B, IsdA, Spa) proteins was observed upon protease deletion. Collectively, our findings provide a unique insight into the progression of CA-MRSA infections, and the role of secreted proteolytic enzymes." @default.
- W2094667070 created "2016-06-24" @default.
- W2094667070 creator A5016887105 @default.
- W2094667070 creator A5023546837 @default.
- W2094667070 creator A5047809253 @default.
- W2094667070 creator A5059988634 @default.
- W2094667070 creator A5064142929 @default.
- W2094667070 creator A5066755414 @default.
- W2094667070 creator A5076509401 @default.
- W2094667070 creator A5089324530 @default.
- W2094667070 date "2012-12-11" @default.
- W2094667070 modified "2023-10-14" @default.
- W2094667070 title "Extracellular proteases are key mediators of<scp><i>S</i></scp><i>taphylococcus aureus</i>virulence via the global modulation of virulence‐determinant stability" @default.
- W2094667070 cites W1509061282 @default.
- W2094667070 cites W1522561094 @default.
- W2094667070 cites W1543460442 @default.
- W2094667070 cites W1964447068 @default.
- W2094667070 cites W1977786353 @default.
- W2094667070 cites W1989998611 @default.
- W2094667070 cites W1992216729 @default.
- W2094667070 cites W1996527079 @default.
- W2094667070 cites W2001203391 @default.
- W2094667070 cites W2002167513 @default.
- W2094667070 cites W2005636153 @default.
- W2094667070 cites W2006840128 @default.
- W2094667070 cites W2012291430 @default.
- W2094667070 cites W2013319822 @default.
- W2094667070 cites W2014399796 @default.
- W2094667070 cites W2017292954 @default.
- W2094667070 cites W2025013124 @default.
- W2094667070 cites W2027426725 @default.
- W2094667070 cites W2035585086 @default.
- W2094667070 cites W2039721978 @default.
- W2094667070 cites W2041628594 @default.
- W2094667070 cites W2042984912 @default.
- W2094667070 cites W2044206614 @default.
- W2094667070 cites W2044750677 @default.
- W2094667070 cites W2046444307 @default.
- W2094667070 cites W2046700055 @default.
- W2094667070 cites W2070578416 @default.
- W2094667070 cites W2073127340 @default.
- W2094667070 cites W2073228865 @default.
- W2094667070 cites W2074195293 @default.
- W2094667070 cites W2076168807 @default.
- W2094667070 cites W2081630781 @default.
- W2094667070 cites W2083596287 @default.
- W2094667070 cites W2085432374 @default.
- W2094667070 cites W2085780766 @default.
- W2094667070 cites W2087906986 @default.
- W2094667070 cites W2089957746 @default.
- W2094667070 cites W2091589827 @default.
- W2094667070 cites W2092006645 @default.
- W2094667070 cites W2092628272 @default.
- W2094667070 cites W2095464022 @default.
- W2094667070 cites W2097493383 @default.
- W2094667070 cites W2098533340 @default.
- W2094667070 cites W2098786490 @default.
- W2094667070 cites W2099936473 @default.
- W2094667070 cites W2100345001 @default.
- W2094667070 cites W2100925647 @default.
- W2094667070 cites W2102361871 @default.
- W2094667070 cites W2103342359 @default.
- W2094667070 cites W2104236199 @default.
- W2094667070 cites W2105466259 @default.
- W2094667070 cites W2114161982 @default.
- W2094667070 cites W2119407884 @default.
- W2094667070 cites W2119793479 @default.
- W2094667070 cites W2121452572 @default.
- W2094667070 cites W2122909249 @default.
- W2094667070 cites W2125829263 @default.
- W2094667070 cites W2126378019 @default.
- W2094667070 cites W2126812384 @default.
- W2094667070 cites W2129016377 @default.
- W2094667070 cites W2129202443 @default.
- W2094667070 cites W2131316809 @default.
- W2094667070 cites W2134829583 @default.
- W2094667070 cites W2138113489 @default.
- W2094667070 cites W2138332871 @default.
- W2094667070 cites W2143527951 @default.
- W2094667070 cites W2144017255 @default.
- W2094667070 cites W2145489399 @default.
- W2094667070 cites W2147200488 @default.
- W2094667070 cites W2148792925 @default.
- W2094667070 cites W2149299339 @default.
- W2094667070 cites W2158808001 @default.
- W2094667070 cites W2162202488 @default.
- W2094667070 cites W2162998751 @default.
- W2094667070 cites W2170205901 @default.
- W2094667070 cites W2314649741 @default.
- W2094667070 cites W4296216849 @default.
- W2094667070 cites W971786142 @default.
- W2094667070 doi "https://doi.org/10.1002/mbo3.55" @default.
- W2094667070 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3584211" @default.
- W2094667070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23233325" @default.
- W2094667070 hasPublicationYear "2012" @default.
- W2094667070 type Work @default.
- W2094667070 sameAs 2094667070 @default.
- W2094667070 citedByCount "138" @default.