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• W2094681483 abstract "Mouse spermatozoa were capacitated and acrosome reacted by incubation for 30 min to 6 h in modified Tyrode's medium (T6) and in cGMP. The fertilization rates of eggs microinjected with a spermatozoon from samples incubated for 30 min, 2 h, and 6 h in T6 and in cGMP were 36%, 34%, 29%, and 43%, respectively. These rates were not correlated to the percentage of acrosome-reacted spermatozoa identified after these treatments. The lack of association could be explained by the selection of an increased proportion of acrosome-reacted spermatozoa actually used for microinjection, particularly in samples incubated for 30 min and 2 h in T6. Both acrosome-intact and acrosome-reacted spermatozoa were identified under the zona in the eggs which failed to fertilize. Fertilized eggs developed at very high rates to the blastocyst stage in vitro." @default.
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• W2094681483 date "1989-06-01" @default.
• W2094681483 modified "2023-09-23" @default.
• W2094681483 title "Fertilization and development of mouse eggs injected under the zona pellucida with single spermatozoa treated to induce the acrosome reaction" @default.
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• W2094681483 doi "https://doi.org/10.1002/mrd.1120230210" @default.
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