FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094691534> ?p ?o ?g. }

• W2094691534 endingPage "875" @default.
• W2094691534 startingPage "869" @default.
• W2094691534 abstract "Abstract: The objectives of the present study were to validate the presence of cytoplasmic and membrane-associated pools of choline acetyltransferase (ChAT) in rat brain synaptosomes, and to evaluate inhibition of these different forms of the enzyme by the nitrogen mustard analogue of choline, choline mustard aziridinium ion (ChM Az). The relative distribution of ChAT and lactate dehydrogenase (LDH) was followed in subfractions of synaptosomes to establish whether ChAT activity associated with salt-washed presynaptic membranes represents membrane-bound protein rather than cytosolic enzyme trapped within undisrupted synaptosomes or revesiculated membrane fragments. The percentage of total synaptosomal ChAT activity (14%) recovered in the final membrane pellet always exceeded that of LDH (6%), lending support to the hypothesis that much of the ChAT associated with the membranes was a membrane bound form of the enzyme. Incubation of purified synaptosomes with ChM Az led to irreversible inhibition of ChAT activity; this loss of enzyme activity could not be accounted for by lysis of nerve terminals during incubation in the presence of the mustard analogue. Subfractionation of the ChM Az-treated nerve terminals revealed that the membrane-bound form of ChAT was inhibited to the greatest extent, followed by the ionically membrane-associated enzyme, with the activity of the water-solubilized enzyme not differing significantly from control. Preparation of the synaptosomal ChAT subfractions from untreated nerve terminals prior to incubation with varying concentrations of ChM Az or naphthylvinylpyridine revealed that under these conditions water-solubilized, ionically membrane-associated, and detergent-solubilized membrane-bound pools of ChAT were not differentially inhibited by either compound. This study suggests that choline mustard transported on the high-affinity choline carrier may be directed toward a membrane-associated form of ChAT." @default.
• W2094691534 created "2016-06-24" @default.
• W2094691534 creator A5020763627 @default.
• W2094691534 date "1989-03-01" @default.
• W2094691534 modified "2023-09-23" @default.
• W2094691534 title "Synaptosomal Membrane-Bound Choline Acetyltransferase Is Most Sensitive to Inhibition by Choline Mustard" @default.
• W2094691534 cites W1566356772 @default.
• W2094691534 cites W1606700480 @default.
• W2094691534 cites W166154308 @default.
• W2094691534 cites W1775749144 @default.
• W2094691534 cites W1980333263 @default.
• W2094691534 cites W1991566402 @default.
• W2094691534 cites W1994720969 @default.
• W2094691534 cites W2003412553 @default.
• W2094691534 cites W2009133656 @default.
• W2094691534 cites W2016246053 @default.
• W2094691534 cites W2019559857 @default.
• W2094691534 cites W2028506450 @default.
• W2094691534 cites W2037675834 @default.
• W2094691534 cites W2045104885 @default.
• W2094691534 cites W2051550116 @default.
• W2094691534 cites W2060882348 @default.
• W2094691534 cites W2065023072 @default.
• W2094691534 cites W2074807479 @default.
• W2094691534 cites W2076963257 @default.
• W2094691534 cites W2078130443 @default.
• W2094691534 cites W2084991109 @default.
• W2094691534 cites W2094404235 @default.
• W2094691534 cites W2102224439 @default.
• W2094691534 cites W2107277845 @default.
• W2094691534 cites W2121405461 @default.
• W2094691534 cites W2125629639 @default.
• W2094691534 cites W2131990375 @default.
• W2094691534 cites W2139214499 @default.
• W2094691534 cites W2141362321 @default.
• W2094691534 cites W2168662313 @default.
• W2094691534 cites W2236626078 @default.
• W2094691534 cites W2323044680 @default.
• W2094691534 cites W2324156447 @default.
• W2094691534 cites W2752712129 @default.
• W2094691534 doi "https://doi.org/10.1111/j.1471-4159.1989.tb02535.x" @default.
• W2094691534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2918313" @default.
• W2094691534 hasPublicationYear "1989" @default.
• W2094691534 type Work @default.
• W2094691534 sameAs 2094691534 @default.
• W2094691534 citedByCount "31" @default.
• W2094691534 countsByYear W20946915342013 @default.
• W2094691534 countsByYear W20946915342014 @default.
• W2094691534 countsByYear W20946915342016 @default.
• W2094691534 countsByYear W20946915342021 @default.
• W2094691534 countsByYear W20946915342022 @default.
• W2094691534 crossrefType "journal-article" @default.
• W2094691534 hasAuthorship W2094691534A5020763627 @default.
• W2094691534 hasConcept C181199279 @default.
• W2094691534 hasConcept C185592680 @default.
• W2094691534 hasConcept C198399640 @default.
• W2094691534 hasConcept C25642318 @default.
• W2094691534 hasConcept C2775910092 @default.
• W2094691534 hasConcept C2778921374 @default.
• W2094691534 hasConcept C2781123555 @default.
• W2094691534 hasConcept C28212737 @default.
• W2094691534 hasConcept C41625074 @default.
• W2094691534 hasConcept C55493867 @default.
• W2094691534 hasConcept C57409179 @default.
• W2094691534 hasConcept C86803240 @default.
• W2094691534 hasConcept C98274493 @default.
• W2094691534 hasConceptScore W2094691534C181199279 @default.
• W2094691534 hasConceptScore W2094691534C185592680 @default.
• W2094691534 hasConceptScore W2094691534C198399640 @default.
• W2094691534 hasConceptScore W2094691534C25642318 @default.
• W2094691534 hasConceptScore W2094691534C2775910092 @default.
• W2094691534 hasConceptScore W2094691534C2778921374 @default.
• W2094691534 hasConceptScore W2094691534C2781123555 @default.
• W2094691534 hasConceptScore W2094691534C28212737 @default.
• W2094691534 hasConceptScore W2094691534C41625074 @default.
• W2094691534 hasConceptScore W2094691534C55493867 @default.
• W2094691534 hasConceptScore W2094691534C57409179 @default.
• W2094691534 hasConceptScore W2094691534C86803240 @default.
• W2094691534 hasConceptScore W2094691534C98274493 @default.
• W2094691534 hasIssue "3" @default.
• W2094691534 hasLocation W20946915341 @default.
• W2094691534 hasLocation W20946915342 @default.
• W2094691534 hasOpenAccess W2094691534 @default.
• W2094691534 hasPrimaryLocation W20946915341 @default.
• W2094691534 hasRelatedWork W1552833558 @default.
• W2094691534 hasRelatedWork W1905237071 @default.
• W2094691534 hasRelatedWork W2001617195 @default.
• W2094691534 hasRelatedWork W2039726498 @default.
• W2094691534 hasRelatedWork W2045149768 @default.
• W2094691534 hasRelatedWork W2094691534 @default.
• W2094691534 hasRelatedWork W2269570030 @default.
• W2094691534 hasRelatedWork W2361542537 @default.
• W2094691534 hasRelatedWork W2409021185 @default.
• W2094691534 hasRelatedWork W2052302874 @default.
• W2094691534 hasVolume "52" @default.
• W2094691534 isParatext "false" @default.
• W2094691534 isRetracted "false" @default.
• W2094691534 magId "2094691534" @default.

• next