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- W2094700568 abstract "A bacterial strain Pseudomonas sp. Zjwp-14 was screened out from soil, and used for the biotransformation of DL-2-amino-Δ <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sup> -thiazoline-4-carbonic acid to L-cysteine. This strain was identified based on its morphological, physiological characterization, 16S rDNA sequence determination and phylogenetic analysis. The bioconversion process was optimized by examining some key factors including cell cultivation time, buffer pH, metal ions, aeration (anaerobic/aerobic) and substrate concentration. L-cysteine productivity reached 4.24 g·1 <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>-1</sup> in 6 h under optimal conditions: cells were cultivated for 12 h, and then resuspended in pH 8.0 phosphate buffer contationing 10 g·1 <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>-1</sup> in of DL-ATC as substrate, with the addition of 2 mM MnSO <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>4</sub> ·4H <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> O, incubated in still culture flashing with N <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> atmosphere at 42 oC for 6 h. The results suggested that Pseudomonas sp. Zjwp-14, a bacterial isolate, has applied potential for enzymatic production of L-cysteine." @default.
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- W2094700568 date "2010-06-01" @default.
- W2094700568 modified "2023-09-28" @default.
- W2094700568 title "Biotransformation of DL-2-Amino-Delta²-Thiazoline-4-Carbonic Acid to L-Cysteine Using a Newly Isolated Pseudomonas sp. Zjwp-14" @default.
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- W2094700568 doi "https://doi.org/10.1109/icbbe.2010.5517181" @default.
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