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- W2094766690 abstract "A sensitive method for measuring nuclear volumes with a Coulter counter is described. It has been applied to the digestion of chicken erythrocyte nuclei by micrococcal nuclease and DNase I. Early in digestion, micrococcal nuclease induced a 20% increase in the effective spherical volume of the nuclei, followed by a gradual reduction. At the peak of nuclear swelling, about 17% of the chromatin was soluble after lysis and its average chain length was about 18 kilobase pairs (kb). DNase I digestion did not give rise to a corresponding expansion of the nuclei. Several preparation conditions, including the treatment of nuclei with 0.2% Triton X-100, led to a loss of the expansion effect upon subsequent micrococcal nuclease digestion. The results support the domain theory of higher order chromatin structure. In the context of this model, the observed maximum nuclear expansion correlates with an average of one nuclease scission per domain." @default.
- W2094766690 created "2016-06-24" @default.
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- W2094766690 date "1982-07-01" @default.
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- W2094766690 title "Expansion of chicken erythrocyte nuclei upon limited micrococcal nuclease digestion Correlation with higher order chromatin structure" @default.
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- W2094766690 doi "https://doi.org/10.1016/0014-4827(82)90156-2" @default.
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