Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094800522> ?p ?o ?g. }
- W2094800522 endingPage "1022" @default.
- W2094800522 startingPage "1012" @default.
- W2094800522 abstract "Coronary vessel development depends on a subpopulation of epicardial cells that undergo epithelial to mesenchymal transformation (EMT) and invade the subepicardial space and myocardium. These cells form the smooth muscle of the vessels and fibroblasts, but the mechanisms that regulate these processes are poorly understood. Mice lacking the Type III Transforming Growth Factor β Receptor (TGFβR3) die by E14.5 due to failed coronary vessel development accompanied by reduced epicardial cell invasion. BMP2 signals via TGFβR3 emphasizing the importance of determining the relative contributions of the canonical BMP signaling pathway and TGFβR3-dependent signaling to BMP2 responsiveness. Here we examined the role of TGFβR3 in BMP2 signaling in epicardial cells. Whereas TGFβ induced loss of epithelial character and smooth muscle differentiation, BMP2 induced an ALK3-dependent loss of epithelial character and modestly inhibited TGFβ-stimulated differentiation. Tgfbr3−/− cells respond to BMP2 indicating that TGFβR3 is not required. However, Tgfbr3−/− cells show decreased invasion in response to BMP2 and overexpression of TGFβR3 in Tgfbr3−/− cells rescued invasion. Invasion was dependent on ALK5, ALK2, ALK3, and Smad4. Expression of TGFβR3 lacking the 3 C-terminal amino acids required to interact with the scaffolding protein GIPC (GAIP-interacting protein, C terminus) did not rescue. Knockdown of GIPC in Tgfbr3+/+ or Tgfbr3−/− cells rescued with TGFβR3 decreased BMP2-stimulated invasion confirming a requirement for TGFβR3/GIPC interaction. Our results reveal the relative roles of TGFβR3-dependent and TGFβR3-independent signaling in the actions of BMP2 on epicardial cell behavior and demonstrate the critical role of TGFβR3 in mediating BMP2-stimulated invasion." @default.
- W2094800522 created "2016-06-24" @default.
- W2094800522 creator A5019787157 @default.
- W2094800522 creator A5024379051 @default.
- W2094800522 creator A5048177881 @default.
- W2094800522 creator A5053271274 @default.
- W2094800522 creator A5055034090 @default.
- W2094800522 creator A5065411046 @default.
- W2094800522 creator A5074432620 @default.
- W2094800522 creator A5083290503 @default.
- W2094800522 date "2012-05-01" @default.
- W2094800522 modified "2023-09-26" @default.
- W2094800522 title "BMP2 signals loss of epithelial character in epicardial cells but requires the Type III TGFβ receptor to promote invasion" @default.
- W2094800522 cites W1590052782 @default.
- W2094800522 cites W1965084403 @default.
- W2094800522 cites W1969619516 @default.
- W2094800522 cites W1971510126 @default.
- W2094800522 cites W1971934808 @default.
- W2094800522 cites W1972175557 @default.
- W2094800522 cites W1972535443 @default.
- W2094800522 cites W1974036913 @default.
- W2094800522 cites W1978999523 @default.
- W2094800522 cites W1980615784 @default.
- W2094800522 cites W1980755859 @default.
- W2094800522 cites W1981803039 @default.
- W2094800522 cites W1983111321 @default.
- W2094800522 cites W1988566424 @default.
- W2094800522 cites W1988613013 @default.
- W2094800522 cites W1988832870 @default.
- W2094800522 cites W1990078733 @default.
- W2094800522 cites W2013950588 @default.
- W2094800522 cites W2014047844 @default.
- W2094800522 cites W2017410696 @default.
- W2094800522 cites W2027080314 @default.
- W2094800522 cites W2028919524 @default.
- W2094800522 cites W2032196758 @default.
- W2094800522 cites W2035023552 @default.
- W2094800522 cites W2043664431 @default.
- W2094800522 cites W2049629754 @default.
- W2094800522 cites W2050549161 @default.
- W2094800522 cites W2051155595 @default.
- W2094800522 cites W2052147002 @default.
- W2094800522 cites W2052825248 @default.
- W2094800522 cites W2053884822 @default.
- W2094800522 cites W2072162746 @default.
- W2094800522 cites W2074629755 @default.
- W2094800522 cites W2075396495 @default.
- W2094800522 cites W2079189474 @default.
- W2094800522 cites W2081142371 @default.
- W2094800522 cites W2086953704 @default.
- W2094800522 cites W2087367170 @default.
- W2094800522 cites W2087598595 @default.
- W2094800522 cites W2100164332 @default.
- W2094800522 cites W2109137601 @default.
- W2094800522 cites W2111949892 @default.
- W2094800522 cites W2112718500 @default.
- W2094800522 cites W2116577936 @default.
- W2094800522 cites W2119738187 @default.
- W2094800522 cites W2121613884 @default.
- W2094800522 cites W2122213184 @default.
- W2094800522 cites W2127776747 @default.
- W2094800522 cites W2139707684 @default.
- W2094800522 cites W2146262826 @default.
- W2094800522 cites W2149748854 @default.
- W2094800522 cites W2149831432 @default.
- W2094800522 cites W2154568166 @default.
- W2094800522 cites W2155008946 @default.
- W2094800522 cites W2156714102 @default.
- W2094800522 cites W2157738334 @default.
- W2094800522 cites W2160437429 @default.
- W2094800522 cites W2165243809 @default.
- W2094800522 cites W2165901622 @default.
- W2094800522 cites W2165987163 @default.
- W2094800522 cites W2170563954 @default.
- W2094800522 cites W2316594132 @default.
- W2094800522 cites W4253422011 @default.
- W2094800522 doi "https://doi.org/10.1016/j.cellsig.2011.12.022" @default.
- W2094800522 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3288519" @default.
- W2094800522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22237159" @default.
- W2094800522 hasPublicationYear "2012" @default.
- W2094800522 type Work @default.
- W2094800522 sameAs 2094800522 @default.
- W2094800522 citedByCount "38" @default.
- W2094800522 countsByYear W20948005222012 @default.
- W2094800522 countsByYear W20948005222013 @default.
- W2094800522 countsByYear W20948005222014 @default.
- W2094800522 countsByYear W20948005222015 @default.
- W2094800522 countsByYear W20948005222016 @default.
- W2094800522 countsByYear W20948005222017 @default.
- W2094800522 countsByYear W20948005222018 @default.
- W2094800522 countsByYear W20948005222019 @default.
- W2094800522 countsByYear W20948005222020 @default.
- W2094800522 countsByYear W20948005222021 @default.
- W2094800522 countsByYear W20948005222022 @default.
- W2094800522 countsByYear W20948005222023 @default.
- W2094800522 crossrefType "journal-article" @default.
- W2094800522 hasAuthorship W2094800522A5019787157 @default.
- W2094800522 hasAuthorship W2094800522A5024379051 @default.