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- W2094835612 abstract "After Mandel and Metais (1) had originally described the existence of free circulating nucleic acids in plasma/serum in humans, Stroun et al. (2)(3) demonstrated that part of the free circulating DNA in cancer patients is of tumor origin (4)(5)(6).The quantity of DNA that can be isolated from human plasma/serum is very low and is frequently the limiting factor when a larger marker panel is to be used for genetic characterization of the DNA. In most of the recently published studies, commercially available columns were used for isolation of cell-free plasma/serum DNA, which made DNA isolation fast and allowed it to be standardized. Mandel and Metais (1) found ∼5.4 μg/mL total nucleic acids in the plasma in 10 healthy controls, and Stroun et al. (3) isolated between 150 ng/mL and 12 μg/mL DNA from the plasma of cancer patients. Much less DNA can be isolated with the aforementioned columns, however; we therefore wondered whether there are ways …" @default.
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- W2094835612 date "2005-08-01" @default.
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- W2094835612 title "Improved Method for Isolating Cell-Free DNA" @default.
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- W2094835612 doi "https://doi.org/10.1373/clinchem.2005.051003" @default.
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