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• W2094853570 endingPage "736" @default.
• W2094853570 startingPage "727" @default.
• W2094853570 abstract "tRNA1Gly is the Escherichia coli glycine tRNA specific for GGG codons. A genetic selection for multicopy suppressors of a frameshift mutation has shown that increased levels of wild-type tRNA1Gly causes −1 frameshifting. Analysis of the suppression spectrum of this multicopy suppressor and peptide sequencing of the suppressed protein product showed that it promoted GG doublet decoding at the near-cognate GGA codons. It is proposed that increasing the concentration of the GGG-specific tRNA1Gly relative to the cognate GGA-decoding tRNA2Gly allows the near-cognate tRNA to read GGA codons. Near-cognate decoding of GGA codons by tRNA1Gly can occur by a two-out-of-three reading mechanism, in which only the first two bases of the GGA codon are paired with the anticodon, thus permitting doublet translocations. In mycoplasmas, a single tRNA typically decodes all four triplets of a codon family and introduction of a feature of the Mypoplasma mycoides tRNAGly responsible for non-discriminate decoding, a C at position 32, into the anticodon E. coli tRNA1Gly, enhanced the efficiency of doublet decoding." @default.
• W2094853570 created "2016-06-24" @default.
• W2094853570 creator A5056336149 @default.
• W2094853570 date "1998-06-01" @default.
• W2094853570 modified "2023-09-26" @default.
• W2094853570 title "tRNA imbalance promotes −1 frameshifting via near-cognate decoding" @default.
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• W2094853570 doi "https://doi.org/10.1006/jmbi.1998.1832" @default.
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