FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2094987029> ?p ?o ?g. }

• W2094987029 endingPage "18720" @default.
• W2094987029 startingPage "18711" @default.
• W2094987029 abstract "Protease-activated receptor-2 (PAR-2) is activated when trypsin cleaves its NH(2) terminus to expose a tethered ligand. We previously demonstrated that PAR-2 activates ion channels in pancreatic duct epithelial cells (PDEC). Using real-time optical fluorescent probes, cyan fluorescence protein-Epac1-yellow fluorescence protein for cAMP, PH(PLC-delta1)-enhanced green fluorescent protein for phosphatidylinositol 4,5-bisphosphate, and protein kinase Cgamma (PKCgamma)-C1-yellow fluorescence protein for diacylglycerol, we now define the signaling pathways mediating PAR-2 effect in dog PDEC. Although PAR-2 activation does not stimulate a cAMP increase, it induces phospholipase C to hydrolyze phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and diacylglycerol. Intracellular Ca(2+) mobilization from inositol 1,4,5-trisphosphate-sensitive Ca(2+) stores and a subsequent Ca(2+) influx through store-operated Ca(2+) channels cause a biphasic increase in intracellular Ca(2+) concentration ([Ca(2+)](i)), measured with Indo-1 dye. Single-cell amperometry demonstrated that this increase in [Ca(2+)](i) in turn causes a biphasic increase in exocytosis. A protein kinase assay revealed that trypsin also activates PKC isozymes to stimulate additional exocytosis. Paralleling the increased exocytosis, mucin secretion from PDEC was also induced by trypsin or the PAR-2 activating peptide. Consistent with the serosal localization of PAR-2, 1 microm luminal trypsin did not induce exocytosis in polarized PDEC monolayers; on the other hand, 10 microm trypsin at 37 degrees C damaged the epithelial barrier sufficiently so that it could reach and activate the serosal PAR-2 to stimulate exocytosis. Thus, in PDEC, PAR-2 activation increases [Ca(2+)](i) and activates PKC to stimulate exocytosis and mucin secretion. These functions may mediate the reported protective role of PAR-2 in different models of pancreatitis." @default.
• W2094987029 created "2016-06-24" @default.
• W2094987029 creator A5007420474 @default.
• W2094987029 creator A5009998245 @default.
• W2094987029 creator A5012052165 @default.
• W2094987029 creator A5022553207 @default.
• W2094987029 creator A5045163049 @default.
• W2094987029 creator A5049507794 @default.
• W2094987029 creator A5064243306 @default.
• W2094987029 creator A5083790649 @default.
• W2094987029 creator A5091483096 @default.
• W2094987029 date "2008-07-01" @default.
• W2094987029 modified "2023-09-28" @default.
• W2094987029 title "Protease-activated Receptor-2 Increases Exocytosis via Multiple Signal Transduction Pathways in Pancreatic Duct Epithelial Cells" @default.
• W2094987029 cites W145763054 @default.
• W2094987029 cites W1861874461 @default.
• W2094987029 cites W1963938486 @default.
• W2094987029 cites W1965081579 @default.
• W2094987029 cites W1971748110 @default.
• W2094987029 cites W1975441923 @default.
• W2094987029 cites W1976028390 @default.
• W2094987029 cites W1989903344 @default.
• W2094987029 cites W1995347602 @default.
• W2094987029 cites W1999015815 @default.
• W2094987029 cites W2005413789 @default.
• W2094987029 cites W2026412696 @default.
• W2094987029 cites W2037583192 @default.
• W2094987029 cites W2041762952 @default.
• W2094987029 cites W2052037106 @default.
• W2094987029 cites W2057043339 @default.
• W2094987029 cites W2076411681 @default.
• W2094987029 cites W2093881154 @default.
• W2094987029 cites W2093951002 @default.
• W2094987029 cites W2098609823 @default.
• W2094987029 cites W2101916673 @default.
• W2094987029 cites W2113564677 @default.
• W2094987029 cites W2123505183 @default.
• W2094987029 cites W2141260882 @default.
• W2094987029 cites W2147758647 @default.
• W2094987029 cites W2180780341 @default.
• W2094987029 cites W2260255165 @default.
• W2094987029 cites W2289439614 @default.
• W2094987029 doi "https://doi.org/10.1074/jbc.m801655200" @default.
• W2094987029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2441557" @default.
• W2094987029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18448425" @default.
• W2094987029 hasPublicationYear "2008" @default.
• W2094987029 type Work @default.
• W2094987029 sameAs 2094987029 @default.
• W2094987029 citedByCount "28" @default.
• W2094987029 countsByYear W20949870292012 @default.
• W2094987029 countsByYear W20949870292013 @default.
• W2094987029 countsByYear W20949870292014 @default.
• W2094987029 countsByYear W20949870292015 @default.
• W2094987029 countsByYear W20949870292016 @default.
• W2094987029 countsByYear W20949870292018 @default.
• W2094987029 countsByYear W20949870292020 @default.
• W2094987029 countsByYear W20949870292021 @default.
• W2094987029 countsByYear W20949870292022 @default.
• W2094987029 crossrefType "journal-article" @default.
• W2094987029 hasAuthorship W2094987029A5007420474 @default.
• W2094987029 hasAuthorship W2094987029A5009998245 @default.
• W2094987029 hasAuthorship W2094987029A5012052165 @default.
• W2094987029 hasAuthorship W2094987029A5022553207 @default.
• W2094987029 hasAuthorship W2094987029A5045163049 @default.
• W2094987029 hasAuthorship W2094987029A5049507794 @default.
• W2094987029 hasAuthorship W2094987029A5064243306 @default.
• W2094987029 hasAuthorship W2094987029A5083790649 @default.
• W2094987029 hasAuthorship W2094987029A5091483096 @default.
• W2094987029 hasBestOaLocation W20949870291 @default.
• W2094987029 hasConcept C153911025 @default.
• W2094987029 hasConcept C163235415 @default.
• W2094987029 hasConcept C175969161 @default.
• W2094987029 hasConcept C185592680 @default.
• W2094987029 hasConcept C195794163 @default.
• W2094987029 hasConcept C2778597717 @default.
• W2094987029 hasConcept C2780610907 @default.
• W2094987029 hasConcept C36020004 @default.
• W2094987029 hasConcept C49039625 @default.
• W2094987029 hasConcept C55493867 @default.
• W2094987029 hasConcept C62478195 @default.
• W2094987029 hasConcept C66135807 @default.
• W2094987029 hasConcept C78297661 @default.
• W2094987029 hasConcept C80631254 @default.
• W2094987029 hasConcept C86803240 @default.
• W2094987029 hasConcept C95444343 @default.
• W2094987029 hasConceptScore W2094987029C153911025 @default.
• W2094987029 hasConceptScore W2094987029C163235415 @default.
• W2094987029 hasConceptScore W2094987029C175969161 @default.
• W2094987029 hasConceptScore W2094987029C185592680 @default.
• W2094987029 hasConceptScore W2094987029C195794163 @default.
• W2094987029 hasConceptScore W2094987029C2778597717 @default.
• W2094987029 hasConceptScore W2094987029C2780610907 @default.
• W2094987029 hasConceptScore W2094987029C36020004 @default.
• W2094987029 hasConceptScore W2094987029C49039625 @default.
• W2094987029 hasConceptScore W2094987029C55493867 @default.
• W2094987029 hasConceptScore W2094987029C62478195 @default.
• W2094987029 hasConceptScore W2094987029C66135807 @default.
• W2094987029 hasConceptScore W2094987029C78297661 @default.

• next