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- W2095019237 abstract "Conditions were established for in vitro culture of seminiferous tubules of adult rat testis. Tubules fragments were able to incorporate radioactive amino acids for up to 6 hours of incubation at 32 degrees C in a modified Eagle's minimum media, indicating biosynthetic activity. Addition of D-glucose (11 Mm) increased the incorporation of either [3H] Leucine or [35S] Methionine four-fold in the protein components of seminiferous tubules. Polyclonal antibodies against outer dense fibers (ODF) polypeptides, which represent approximately 30% of the total sperm proteins, immunoprecipitated 5% of the total radioactivity from cultures carried out either in the presence or absence of D-glucose. Moreover, antibodies specific for the 27-30 kilodalton polypeptides of ODF immunoprecipitated 2% of the total radioactivity, showing no differences in the presence and absence of D-glucose. This study indicates that ODF polypeptides can be synthesized in vitro at 32 degrees C with and without D-glucose." @default.
- W2095019237 created "2016-06-24" @default.
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- W2095019237 date "1991-01-01" @default.
- W2095019237 modified "2023-09-23" @default.
- W2095019237 title "In vitro biosynthesis of rat sperm outer dense fiber components" @default.
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- W2095019237 doi "https://doi.org/10.1016/0024-3205(91)90327-8" @default.
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