Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095059123> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2095059123 endingPage "92" @default.
- W2095059123 startingPage "85" @default.
- W2095059123 abstract "The egg white glycoprotein, ovalbumin, is known to be microheterogeneous as a result of its varied glycan content. The use of 1,4-diaminobutane (DAB) as a buffer additive has been shown to be key in the high-resolution capillary electrophoretic separation of “glycoforms” of this protein [Anal. Biochem. 205 (1992) 115]. Although a separation buffer consisting of 100 mM borate and 1 mM DAB allowed for adequate separation of ovalbumin glycoforms, prolonged separation times of 35–45 min were undesirable. In the present study, the α,ω-bis-quaternary ammonium alkanes, hexamethonium bromide (C6MetBr), hexamethonium chloride (C6MetCl) and decamethonium bromide (C10MetBr) were tested as buffer additives for their effectiveness in the separation of ovalbumin glycoforms. Where 1 mM DAB gave optimal separation in ca. 45 min, 100 μM C6MetCl or C10MetBr yielded comparable resolution in less than 20 min. Results with the C10MetBr were better than those obtained with C6MetBr, indicating that there may be a correlation between effectiveness and alkyl chain length. Use of the chloride salt of C6Met afforded the same resolution as the bromide salt in slightly shorter analysis time. The rank order for their effectiveness was found to be C10MetBr > C6MetCl > C6MetBr > DAB. These results allow for speculation on the mode through which these additives exert their effect on resolution. Included in these are additive-wall coating interactions, protein-additive interactions, protein-wall interactions or any combination of these." @default.
- W2095059123 created "2016-06-24" @default.
- W2095059123 creator A5031563436 @default.
- W2095059123 creator A5054911795 @default.
- W2095059123 creator A5057920575 @default.
- W2095059123 creator A5058181679 @default.
- W2095059123 date "1994-09-01" @default.
- W2095059123 modified "2023-10-10" @default.
- W2095059123 title "α,ω-Bis-quaternary ammonium alkanes as effective buffer additives for enhanced capillary electrophoretic separation of glycoproteins" @default.
- W2095059123 cites W1485462441 @default.
- W2095059123 cites W1496656839 @default.
- W2095059123 cites W1529190675 @default.
- W2095059123 cites W1564905326 @default.
- W2095059123 cites W1625540616 @default.
- W2095059123 cites W1977967214 @default.
- W2095059123 cites W1985529268 @default.
- W2095059123 cites W1985683244 @default.
- W2095059123 cites W1994702378 @default.
- W2095059123 cites W1998222484 @default.
- W2095059123 cites W1999173093 @default.
- W2095059123 cites W2001800684 @default.
- W2095059123 cites W2022904374 @default.
- W2095059123 cites W2025907940 @default.
- W2095059123 cites W2032434056 @default.
- W2095059123 cites W2056749665 @default.
- W2095059123 cites W2058925917 @default.
- W2095059123 cites W2063314780 @default.
- W2095059123 cites W2068473218 @default.
- W2095059123 cites W2113630335 @default.
- W2095059123 cites W2160949470 @default.
- W2095059123 cites W2167416243 @default.
- W2095059123 cites W96640158 @default.
- W2095059123 doi "https://doi.org/10.1016/0021-9673(94)80055-3" @default.
- W2095059123 hasPublicationYear "1994" @default.
- W2095059123 type Work @default.
- W2095059123 sameAs 2095059123 @default.
- W2095059123 citedByCount "48" @default.
- W2095059123 crossrefType "journal-article" @default.
- W2095059123 hasAuthorship W2095059123A5031563436 @default.
- W2095059123 hasAuthorship W2095059123A5054911795 @default.
- W2095059123 hasAuthorship W2095059123A5057920575 @default.
- W2095059123 hasAuthorship W2095059123A5058181679 @default.
- W2095059123 hasConcept C138268822 @default.
- W2095059123 hasConcept C154945302 @default.
- W2095059123 hasConcept C178790620 @default.
- W2095059123 hasConcept C179104552 @default.
- W2095059123 hasConcept C185592680 @default.
- W2095059123 hasConcept C203014093 @default.
- W2095059123 hasConcept C2776300020 @default.
- W2095059123 hasConcept C2776371256 @default.
- W2095059123 hasConcept C2776946954 @default.
- W2095059123 hasConcept C2778695967 @default.
- W2095059123 hasConcept C2780263894 @default.
- W2095059123 hasConcept C2900643 @default.
- W2095059123 hasConcept C41008148 @default.
- W2095059123 hasConcept C43617362 @default.
- W2095059123 hasConcept C86803240 @default.
- W2095059123 hasConcept C8891405 @default.
- W2095059123 hasConceptScore W2095059123C138268822 @default.
- W2095059123 hasConceptScore W2095059123C154945302 @default.
- W2095059123 hasConceptScore W2095059123C178790620 @default.
- W2095059123 hasConceptScore W2095059123C179104552 @default.
- W2095059123 hasConceptScore W2095059123C185592680 @default.
- W2095059123 hasConceptScore W2095059123C203014093 @default.
- W2095059123 hasConceptScore W2095059123C2776300020 @default.
- W2095059123 hasConceptScore W2095059123C2776371256 @default.
- W2095059123 hasConceptScore W2095059123C2776946954 @default.
- W2095059123 hasConceptScore W2095059123C2778695967 @default.
- W2095059123 hasConceptScore W2095059123C2780263894 @default.
- W2095059123 hasConceptScore W2095059123C2900643 @default.
- W2095059123 hasConceptScore W2095059123C41008148 @default.
- W2095059123 hasConceptScore W2095059123C43617362 @default.
- W2095059123 hasConceptScore W2095059123C86803240 @default.
- W2095059123 hasConceptScore W2095059123C8891405 @default.
- W2095059123 hasIssue "1" @default.
- W2095059123 hasLocation W20950591231 @default.
- W2095059123 hasOpenAccess W2095059123 @default.
- W2095059123 hasPrimaryLocation W20950591231 @default.
- W2095059123 hasRelatedWork W1964674919 @default.
- W2095059123 hasRelatedWork W1968234338 @default.
- W2095059123 hasRelatedWork W2002936791 @default.
- W2095059123 hasRelatedWork W2007535992 @default.
- W2095059123 hasRelatedWork W2036175167 @default.
- W2095059123 hasRelatedWork W2079537492 @default.
- W2095059123 hasRelatedWork W2286986362 @default.
- W2095059123 hasRelatedWork W263784416 @default.
- W2095059123 hasRelatedWork W284386456 @default.
- W2095059123 hasRelatedWork W3118313920 @default.
- W2095059123 hasVolume "680" @default.
- W2095059123 isParatext "false" @default.
- W2095059123 isRetracted "false" @default.
- W2095059123 magId "2095059123" @default.
- W2095059123 workType "article" @default.