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- W2095087180 abstract "Methods of preparing tissues and the compromises necessary in the application of histochemistry to electron microscopy are reviewed. Three methods are described for demonstrating succinic dehydrogenase with the electron microscope and the results are compared with those for standard cytochemical procedures. A cholinesterase-like enzyme may be localized in striated muscle by a metal salt method. Azo-dye histochemical methods performed in vivo for the demonstration of alkaline phosphatase activity with the electron microscope reveal that the final dye product is picked up by the duodenal epithelium by pinocytosis. The dye particles are transported through the cells and discharged at the lateral cell margins. For the successful demonstration of alkaline phosphatase activity, prefixation is necessary to prevent the pinocytosis without destroying the enzyme." @default.
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- W2095087180 date "1959-01-01" @default.
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- W2095087180 title "The demonstration with the electron microscope of the end-products of histochemical reactions in relation to the fine structure of cells*1" @default.
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- W2095087180 doi "https://doi.org/10.1016/0014-4827(59)90235-6" @default.
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