Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095115428> ?p ?o ?g. }
- W2095115428 endingPage "1049" @default.
- W2095115428 startingPage "1025" @default.
- W2095115428 abstract "Heparinase I (Hep I) can efficiently depolymerize heparin and heparin sulfate to oligosaccharides or unsaturated disaccharides, which resulted in loss of physiological function such as blood coagulation. In order to realize the immobilization of Hep I on chitin carriers, we cloned Hep I with the chitin binding domain (ChBD) as a chitin-affinity tag, and the Small Ubiquitin-like MOdifier (SUMO) linker as a solvation enhancer in different fusion sequence. DNA and protein gels suggested that 4 kinds of recombinants were successfully constructed and expressed in Escherichia coli (E. coli). And the triple functional heparinases isolated from cell lysate could be efficiently purified by chitin beads. After optimizing fermentation conditions, it gave the specific enzyme activities of 1.88 ± 0.11, 3.69 ± 0.45, 3.44 ± 0.38, and 2.73 ± 0.29 IU/mg total proteins for ChBD-Hep I, ChBD-SUMO-Hep I, SUMO-ChBD-Hep I, and ChBD-Hep I-SUMO, respectively, with unfractionated heparin as substrate. The optimal reaction temperature and pH were determined to be 30 °C and 7.0 for all the fusion enzymes. ChBD-SUMO-Hep I exhibited the maximum half-life (48 min) at 30 °C and best thermo-stability under 15–50 °C. All the fusion enzymes showed broad pH-stability in the range of 5.4–9.0." @default.
- W2095115428 created "2016-06-24" @default.
- W2095115428 creator A5013364691 @default.
- W2095115428 creator A5067679427 @default.
- W2095115428 date "2001-11-01" @default.
- W2095115428 modified "2023-10-02" @default.
- W2095115428 title "PHARMACOLOGY OF LOW MOLECULAR WEIGHT HARPARINS" @default.
- W2095115428 cites W154118115 @default.
- W2095115428 cites W1559267373 @default.
- W2095115428 cites W1581187193 @default.
- W2095115428 cites W168997661 @default.
- W2095115428 cites W1782024978 @default.
- W2095115428 cites W1964506112 @default.
- W2095115428 cites W1969683016 @default.
- W2095115428 cites W1975046020 @default.
- W2095115428 cites W1975322829 @default.
- W2095115428 cites W1989266331 @default.
- W2095115428 cites W1996040171 @default.
- W2095115428 cites W2006103715 @default.
- W2095115428 cites W2013984355 @default.
- W2095115428 cites W2021206861 @default.
- W2095115428 cites W2023886702 @default.
- W2095115428 cites W2029729589 @default.
- W2095115428 cites W2032512409 @default.
- W2095115428 cites W2032757997 @default.
- W2095115428 cites W2034586141 @default.
- W2095115428 cites W2035959805 @default.
- W2095115428 cites W2041709488 @default.
- W2095115428 cites W2049276614 @default.
- W2095115428 cites W2050364432 @default.
- W2095115428 cites W2051836214 @default.
- W2095115428 cites W2058352743 @default.
- W2095115428 cites W2063207586 @default.
- W2095115428 cites W2064442519 @default.
- W2095115428 cites W2065624758 @default.
- W2095115428 cites W2070676143 @default.
- W2095115428 cites W2073330986 @default.
- W2095115428 cites W2074442948 @default.
- W2095115428 cites W2075196907 @default.
- W2095115428 cites W2078335793 @default.
- W2095115428 cites W2083258650 @default.
- W2095115428 cites W2085060329 @default.
- W2095115428 cites W2093363651 @default.
- W2095115428 cites W2094804000 @default.
- W2095115428 cites W2094944787 @default.
- W2095115428 cites W2099748193 @default.
- W2095115428 cites W2110973089 @default.
- W2095115428 cites W2123442443 @default.
- W2095115428 cites W2124787187 @default.
- W2095115428 cites W2127086872 @default.
- W2095115428 cites W2128905954 @default.
- W2095115428 cites W2138845401 @default.
- W2095115428 cites W2145308085 @default.
- W2095115428 cites W2145710740 @default.
- W2095115428 cites W2149599987 @default.
- W2095115428 cites W2154585516 @default.
- W2095115428 cites W2166137806 @default.
- W2095115428 cites W2168503421 @default.
- W2095115428 cites W2171119585 @default.
- W2095115428 cites W2196887485 @default.
- W2095115428 cites W2313868127 @default.
- W2095115428 cites W2316460201 @default.
- W2095115428 cites W2329311416 @default.
- W2095115428 cites W2333983635 @default.
- W2095115428 cites W2338618131 @default.
- W2095115428 cites W2340468754 @default.
- W2095115428 cites W2342200163 @default.
- W2095115428 cites W2395098672 @default.
- W2095115428 cites W2969335355 @default.
- W2095115428 cites W2973596107 @default.
- W2095115428 cites W2992861829 @default.
- W2095115428 cites W367384902 @default.
- W2095115428 cites W4211253301 @default.
- W2095115428 cites W4300616926 @default.
- W2095115428 cites W4317929375 @default.
- W2095115428 cites W4323283745 @default.
- W2095115428 cites W4324114342 @default.
- W2095115428 cites W4324262398 @default.
- W2095115428 cites W74018345 @default.
- W2095115428 doi "https://doi.org/10.1016/s0733-8627(05)70233-9" @default.
- W2095115428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11762273" @default.
- W2095115428 hasPublicationYear "2001" @default.
- W2095115428 type Work @default.
- W2095115428 sameAs 2095115428 @default.
- W2095115428 citedByCount "21" @default.
- W2095115428 countsByYear W20951154282012 @default.
- W2095115428 countsByYear W20951154282013 @default.
- W2095115428 countsByYear W20951154282016 @default.
- W2095115428 countsByYear W20951154282017 @default.
- W2095115428 countsByYear W20951154282021 @default.
- W2095115428 countsByYear W20951154282023 @default.
- W2095115428 crossrefType "journal-article" @default.
- W2095115428 hasAuthorship W2095115428A5013364691 @default.
- W2095115428 hasAuthorship W2095115428A5067679427 @default.
- W2095115428 hasConcept C104317684 @default.
- W2095115428 hasConcept C123894998 @default.
- W2095115428 hasConcept C181199279 @default.
- W2095115428 hasConcept C185592680 @default.