Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095120004> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2095120004 endingPage "8709" @default.
- W2095120004 startingPage "8699" @default.
- W2095120004 abstract "The sequence G37pG36 is present in all tRNA species recognized and methylated by the Escherichia coli modification enzyme tRNA (guanosine-1)methyltransferase. We have examined whether this dinucleotide sequence provides the base specific recognition signal for this enzyme and have assessed the role of the remaining tRNA in recognition. E. coli tRNAHis and yeast tRNAAsp were substituted with G at positions 36 and 37 and were found to be excellent substrates for methylation. This suggested that the general tRNA structure can be specifically bound by the enzyme. In addition, heterologous tRNA species including fully modified tRNA1Leu are excellent inhibitors of tRNA1Leu transcript methylation. Analyses of structural variants of yeast tRNAAsp and E. coli tRNA1Leu demonstrate clearly that the core tertiary structures of tRNA are required for recognition and that G37 must be in the correct position in space relative to important contacts elsewhere in the molecule. This latter conclusion was reached because the addition of one to three stacked base pairs in the anticodon stem of tRNA1Leu dramatically alters activity. In this case, the G37 base is rotated away from the correct position in space relative to other tRNA contact sites. The acceptor stem structure is required for optimal activity since deletion of three or five base pairs is detrimental to activity; however, specific base sequence may not be important because (i) the addition of three stacked base pairs of different sequence had little effect on activity and (ii) heterologous tRNAs with little or no sequence homology in the acceptor stem are excellent substrates. Both poly G and GpG are potent and specific inhibitors of enzyme activity and are minimal substrates which can be methylated, forming m1G. Taken together, these studies suggest that 1MGT can bind the general tRNA structure and that the crucial base-pair contacts are G37 and G36." @default.
- W2095120004 created "2016-06-24" @default.
- W2095120004 creator A5009084800 @default.
- W2095120004 creator A5031345360 @default.
- W2095120004 creator A5048915170 @default.
- W2095120004 creator A5054694568 @default.
- W2095120004 creator A5088580567 @default.
- W2095120004 date "1997-07-01" @default.
- W2095120004 modified "2023-10-17" @default.
- W2095120004 title "Interaction of tRNA with tRNA (Guanosine-1)methyltransferase: Binding Specificity Determinants Involve the Dinucleotide G<sup>36</sup>pG<sup>37</sup> and Tertiary Structure" @default.
- W2095120004 cites W150342077 @default.
- W2095120004 cites W1540963830 @default.
- W2095120004 cites W1552938235 @default.
- W2095120004 cites W1820306814 @default.
- W2095120004 cites W1995416079 @default.
- W2095120004 cites W2029551024 @default.
- W2095120004 cites W2054735031 @default.
- W2095120004 cites W2087748376 @default.
- W2095120004 cites W2103947911 @default.
- W2095120004 cites W2139283159 @default.
- W2095120004 cites W2265837524 @default.
- W2095120004 doi "https://doi.org/10.1021/bi9701538" @default.
- W2095120004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9220956" @default.
- W2095120004 hasPublicationYear "1997" @default.
- W2095120004 type Work @default.
- W2095120004 sameAs 2095120004 @default.
- W2095120004 citedByCount "43" @default.
- W2095120004 countsByYear W20951200042012 @default.
- W2095120004 countsByYear W20951200042013 @default.
- W2095120004 countsByYear W20951200042014 @default.
- W2095120004 countsByYear W20951200042015 @default.
- W2095120004 countsByYear W20951200042016 @default.
- W2095120004 countsByYear W20951200042017 @default.
- W2095120004 countsByYear W20951200042019 @default.
- W2095120004 countsByYear W20951200042020 @default.
- W2095120004 countsByYear W20951200042021 @default.
- W2095120004 countsByYear W20951200042022 @default.
- W2095120004 countsByYear W20951200042023 @default.
- W2095120004 crossrefType "journal-article" @default.
- W2095120004 hasAuthorship W2095120004A5009084800 @default.
- W2095120004 hasAuthorship W2095120004A5031345360 @default.
- W2095120004 hasAuthorship W2095120004A5048915170 @default.
- W2095120004 hasAuthorship W2095120004A5054694568 @default.
- W2095120004 hasAuthorship W2095120004A5088580567 @default.
- W2095120004 hasConcept C104317684 @default.
- W2095120004 hasConcept C153957851 @default.
- W2095120004 hasConcept C185581394 @default.
- W2095120004 hasConcept C185592680 @default.
- W2095120004 hasConcept C203353237 @default.
- W2095120004 hasConcept C2777995097 @default.
- W2095120004 hasConcept C33288867 @default.
- W2095120004 hasConcept C547475151 @default.
- W2095120004 hasConcept C552990157 @default.
- W2095120004 hasConcept C55493867 @default.
- W2095120004 hasConcept C67705224 @default.
- W2095120004 hasConcept C71240020 @default.
- W2095120004 hasConcept C75599170 @default.
- W2095120004 hasConcept C86803240 @default.
- W2095120004 hasConceptScore W2095120004C104317684 @default.
- W2095120004 hasConceptScore W2095120004C153957851 @default.
- W2095120004 hasConceptScore W2095120004C185581394 @default.
- W2095120004 hasConceptScore W2095120004C185592680 @default.
- W2095120004 hasConceptScore W2095120004C203353237 @default.
- W2095120004 hasConceptScore W2095120004C2777995097 @default.
- W2095120004 hasConceptScore W2095120004C33288867 @default.
- W2095120004 hasConceptScore W2095120004C547475151 @default.
- W2095120004 hasConceptScore W2095120004C552990157 @default.
- W2095120004 hasConceptScore W2095120004C55493867 @default.
- W2095120004 hasConceptScore W2095120004C67705224 @default.
- W2095120004 hasConceptScore W2095120004C71240020 @default.
- W2095120004 hasConceptScore W2095120004C75599170 @default.
- W2095120004 hasConceptScore W2095120004C86803240 @default.
- W2095120004 hasIssue "29" @default.
- W2095120004 hasLocation W20951200041 @default.
- W2095120004 hasLocation W20951200042 @default.
- W2095120004 hasOpenAccess W2095120004 @default.
- W2095120004 hasPrimaryLocation W20951200041 @default.
- W2095120004 hasRelatedWork W1562653427 @default.
- W2095120004 hasRelatedWork W1776186831 @default.
- W2095120004 hasRelatedWork W2029498998 @default.
- W2095120004 hasRelatedWork W2039685559 @default.
- W2095120004 hasRelatedWork W2065424596 @default.
- W2095120004 hasRelatedWork W2091022802 @default.
- W2095120004 hasRelatedWork W2095120004 @default.
- W2095120004 hasRelatedWork W2789591804 @default.
- W2095120004 hasRelatedWork W2885883227 @default.
- W2095120004 hasRelatedWork W3016909595 @default.
- W2095120004 hasVolume "36" @default.
- W2095120004 isParatext "false" @default.
- W2095120004 isRetracted "false" @default.
- W2095120004 magId "2095120004" @default.
- W2095120004 workType "article" @default.