Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095157776> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2095157776 endingPage "147" @default.
- W2095157776 startingPage "129" @default.
- W2095157776 abstract "Abstract The chromatographic parameters affectingthe reversed-phase high-performance liquid chromatographic (HPLC) separation of major and modified nucleosides with a μBondapak C 18 column have been studied. This investigation has resulted in the HPLC separation of eighteen nucleosides in a single analysis. The parameters studied include: the mobile phase flow-rate, pH, methanol concentration, column temperature and injection volume. Each parameter was investigated individually to observe the effect on the chromatographic behavior of the nucleosides. The relationships which we have established for the elution of the nucleosides as a function of the respective parameters investigated can be used to predict their separation. From these experiments, the chromatographic conditions for the separation of urinary nucleosides were optimized using both isocratic and step gradient conditions. The step gradient system is more suitable for determining the nucleoside composition of tRNA hydrolysates, and the complete separation of the major ribo- and deoxyribonucleosides can be accomplished. Also, we have studied the storage stability of urinary nucleosides, and have looked for nucleotides and oligonucleotides in normal and cancer patient uring and found none. In addition, we report a rapid isocratic system for the separation of m 2 2 G and t 6 A. A most significant aspect of this research is the determination of the effects of various chromatographic parameters on the reversed-phase HPLC separation of the nucleosides. These findings provide great flexibility in the analysis of nucleosides in that these data form a guide for finding optimal conditions for nucleosides separations. This chromatography is of importance in the accurate determination of tRNA composition, especially to scientists investigating tRNA biosynthesis, function and sequence, and also for investigations on the purity of RNA and DNA isolations, and research on DNA and its modification." @default.
- W2095157776 created "2016-06-24" @default.
- W2095157776 creator A5025537515 @default.
- W2095157776 creator A5070607952 @default.
- W2095157776 creator A5087728571 @default.
- W2095157776 date "1980-01-01" @default.
- W2095157776 modified "2023-09-27" @default.
- W2095157776 title "Chromatography of nucleosides" @default.
- W2095157776 cites W1484592612 @default.
- W2095157776 cites W1534167409 @default.
- W2095157776 cites W1549825490 @default.
- W2095157776 cites W1587374461 @default.
- W2095157776 cites W1800752973 @default.
- W2095157776 cites W1969144323 @default.
- W2095157776 cites W1972788558 @default.
- W2095157776 cites W1979431647 @default.
- W2095157776 cites W198083544 @default.
- W2095157776 cites W1984002239 @default.
- W2095157776 cites W1984995729 @default.
- W2095157776 cites W1988884895 @default.
- W2095157776 cites W1999841594 @default.
- W2095157776 cites W1999925815 @default.
- W2095157776 cites W2000853402 @default.
- W2095157776 cites W2003080750 @default.
- W2095157776 cites W2007195341 @default.
- W2095157776 cites W2011550939 @default.
- W2095157776 cites W2021620619 @default.
- W2095157776 cites W2022886623 @default.
- W2095157776 cites W2025250760 @default.
- W2095157776 cites W2032969861 @default.
- W2095157776 cites W2033571794 @default.
- W2095157776 cites W2041833143 @default.
- W2095157776 cites W2043794845 @default.
- W2095157776 cites W2044886210 @default.
- W2095157776 cites W2051335716 @default.
- W2095157776 cites W2056395050 @default.
- W2095157776 cites W2059763279 @default.
- W2095157776 cites W2062636111 @default.
- W2095157776 cites W2063257535 @default.
- W2095157776 cites W2063915418 @default.
- W2095157776 cites W2066667311 @default.
- W2095157776 cites W2071811494 @default.
- W2095157776 cites W2073034458 @default.
- W2095157776 cites W2080761417 @default.
- W2095157776 cites W2119458962 @default.
- W2095157776 cites W2153646232 @default.
- W2095157776 cites W2315568519 @default.
- W2095157776 cites W2316488470 @default.
- W2095157776 cites W2343729575 @default.
- W2095157776 cites W4235944065 @default.
- W2095157776 cites W87107169 @default.
- W2095157776 doi "https://doi.org/10.1016/s0021-9673(00)88424-1" @default.
- W2095157776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7380928" @default.
- W2095157776 hasPublicationYear "1980" @default.
- W2095157776 type Work @default.
- W2095157776 sameAs 2095157776 @default.
- W2095157776 citedByCount "111" @default.
- W2095157776 countsByYear W20951577762015 @default.
- W2095157776 countsByYear W20951577762019 @default.
- W2095157776 countsByYear W20951577762021 @default.
- W2095157776 crossrefType "journal-article" @default.
- W2095157776 hasAuthorship W2095157776A5025537515 @default.
- W2095157776 hasAuthorship W2095157776A5070607952 @default.
- W2095157776 hasAuthorship W2095157776A5087728571 @default.
- W2095157776 hasConcept C185592680 @default.
- W2095157776 hasConcept C43617362 @default.
- W2095157776 hasConceptScore W2095157776C185592680 @default.
- W2095157776 hasConceptScore W2095157776C43617362 @default.
- W2095157776 hasIssue "1" @default.
- W2095157776 hasLocation W20951577761 @default.
- W2095157776 hasLocation W20951577762 @default.
- W2095157776 hasOpenAccess W2095157776 @default.
- W2095157776 hasPrimaryLocation W20951577761 @default.
- W2095157776 hasRelatedWork W1531601525 @default.
- W2095157776 hasRelatedWork W1990781990 @default.
- W2095157776 hasRelatedWork W2314643286 @default.
- W2095157776 hasRelatedWork W2606768298 @default.
- W2095157776 hasRelatedWork W2606769243 @default.
- W2095157776 hasRelatedWork W2606837276 @default.
- W2095157776 hasRelatedWork W2748952813 @default.
- W2095157776 hasRelatedWork W2899084033 @default.
- W2095157776 hasRelatedWork W2948807893 @default.
- W2095157776 hasRelatedWork W2778153218 @default.
- W2095157776 hasVolume "188" @default.
- W2095157776 isParatext "false" @default.
- W2095157776 isRetracted "false" @default.
- W2095157776 magId "2095157776" @default.
- W2095157776 workType "article" @default.