Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095166475> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2095166475 endingPage "73" @default.
- W2095166475 startingPage "66" @default.
- W2095166475 abstract "Methods probing protein-DNA associations include direct binding titrations and competition binding experiments. For the latter, we present here a simple procedure allowing the quantitative evaluation of dissociation constants. We show that the ratio between the fraction of a DNA probe bound to protein in the absence of competitor and that in the presence of competitor is, at large competitor concentrations, a linear function of the competitor concentration, and we derive equations allowing the dissociation constant for the protein-competitor complex to be evaluated from the slope. We show further that a self-competition experiment, where the DNA probe and competitor are chemically the same species, can be used as a complement to a direct titration to determine the fraction of protein that is correctly folded for specific DNA binding. Thus, such a combination of direct and self-competition titration can be used as a check of the conformational purity of DNA binding proteins." @default.
- W2095166475 created "2016-06-24" @default.
- W2095166475 creator A5052298740 @default.
- W2095166475 date "2011-02-01" @default.
- W2095166475 modified "2023-09-29" @default.
- W2095166475 title "Evaluation of dissociation constants from competition binding experiments based on the relative binding ratio" @default.
- W2095166475 cites W1566822127 @default.
- W2095166475 cites W1850804182 @default.
- W2095166475 cites W1859642 @default.
- W2095166475 cites W1965267927 @default.
- W2095166475 cites W1967405783 @default.
- W2095166475 cites W1968278001 @default.
- W2095166475 cites W1976260744 @default.
- W2095166475 cites W1981358208 @default.
- W2095166475 cites W198399274 @default.
- W2095166475 cites W2000215876 @default.
- W2095166475 cites W2004563262 @default.
- W2095166475 cites W2032363898 @default.
- W2095166475 cites W2034880487 @default.
- W2095166475 cites W2040526720 @default.
- W2095166475 cites W2041701954 @default.
- W2095166475 cites W2054245739 @default.
- W2095166475 cites W2079157510 @default.
- W2095166475 cites W2087234479 @default.
- W2095166475 cites W2117175955 @default.
- W2095166475 cites W2162295076 @default.
- W2095166475 cites W2341283081 @default.
- W2095166475 cites W3024136902 @default.
- W2095166475 cites W3107148387 @default.
- W2095166475 doi "https://doi.org/10.1016/j.ab.2010.09.023" @default.
- W2095166475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20883661" @default.
- W2095166475 hasPublicationYear "2011" @default.
- W2095166475 type Work @default.
- W2095166475 sameAs 2095166475 @default.
- W2095166475 citedByCount "0" @default.
- W2095166475 crossrefType "journal-article" @default.
- W2095166475 hasAuthorship W2095166475A5052298740 @default.
- W2095166475 hasConcept C102931765 @default.
- W2095166475 hasConcept C107824862 @default.
- W2095166475 hasConcept C12554922 @default.
- W2095166475 hasConcept C147789679 @default.
- W2095166475 hasConcept C170493617 @default.
- W2095166475 hasConcept C184866935 @default.
- W2095166475 hasConcept C185592680 @default.
- W2095166475 hasConcept C3019856697 @default.
- W2095166475 hasConcept C32786932 @default.
- W2095166475 hasConcept C51639874 @default.
- W2095166475 hasConcept C552990157 @default.
- W2095166475 hasConcept C55493867 @default.
- W2095166475 hasConcept C74998103 @default.
- W2095166475 hasConcept C86803240 @default.
- W2095166475 hasConceptScore W2095166475C102931765 @default.
- W2095166475 hasConceptScore W2095166475C107824862 @default.
- W2095166475 hasConceptScore W2095166475C12554922 @default.
- W2095166475 hasConceptScore W2095166475C147789679 @default.
- W2095166475 hasConceptScore W2095166475C170493617 @default.
- W2095166475 hasConceptScore W2095166475C184866935 @default.
- W2095166475 hasConceptScore W2095166475C185592680 @default.
- W2095166475 hasConceptScore W2095166475C3019856697 @default.
- W2095166475 hasConceptScore W2095166475C32786932 @default.
- W2095166475 hasConceptScore W2095166475C51639874 @default.
- W2095166475 hasConceptScore W2095166475C552990157 @default.
- W2095166475 hasConceptScore W2095166475C55493867 @default.
- W2095166475 hasConceptScore W2095166475C74998103 @default.
- W2095166475 hasConceptScore W2095166475C86803240 @default.
- W2095166475 hasIssue "1" @default.
- W2095166475 hasLocation W20951664751 @default.
- W2095166475 hasLocation W20951664752 @default.
- W2095166475 hasOpenAccess W2095166475 @default.
- W2095166475 hasPrimaryLocation W20951664751 @default.
- W2095166475 hasRelatedWork W1484657725 @default.
- W2095166475 hasRelatedWork W1532965988 @default.
- W2095166475 hasRelatedWork W1968786854 @default.
- W2095166475 hasRelatedWork W1993391957 @default.
- W2095166475 hasRelatedWork W1997247827 @default.
- W2095166475 hasRelatedWork W1997755437 @default.
- W2095166475 hasRelatedWork W1998627068 @default.
- W2095166475 hasRelatedWork W1999428731 @default.
- W2095166475 hasRelatedWork W2006792964 @default.
- W2095166475 hasRelatedWork W2019417006 @default.
- W2095166475 hasRelatedWork W2029323948 @default.
- W2095166475 hasRelatedWork W2035733725 @default.
- W2095166475 hasRelatedWork W2040007286 @default.
- W2095166475 hasRelatedWork W2056769699 @default.
- W2095166475 hasRelatedWork W2056927170 @default.
- W2095166475 hasRelatedWork W2095150400 @default.
- W2095166475 hasRelatedWork W2101738230 @default.
- W2095166475 hasRelatedWork W2116573367 @default.
- W2095166475 hasRelatedWork W2461793616 @default.
- W2095166475 hasRelatedWork W2912445187 @default.
- W2095166475 hasVolume "409" @default.
- W2095166475 isParatext "false" @default.
- W2095166475 isRetracted "false" @default.
- W2095166475 magId "2095166475" @default.
- W2095166475 workType "article" @default.