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- W2095175046 abstract "To determine nitrogen and carbon uptake in the surface waters of oligotrophic Lake Taupo, New Zealand, we used isotope tracer methods. Time-course measurements of NH 4 + and NO 3 − uptake at near saturating concentration revealed about a fourfold greater uptake during the first hour of incubation relative to averaged 1–6 h uptake rates. This enhanced uptake was most apparent during the first 5–10 min of incubation. Uptake kinetics experiments indicated that the N assimilation rate was generally limited by low ambient concentrations of inorganic N. The half-saturation constants for N uptake were low [Formula: see text] and comparable with ambient levels of NH 4 + and NO 3 − ; NO 3 − uptake was always lower than NH 4 + uptake and was inhibited strongly (~60%) in the presence of ~5 μg NH 4 + -N∙L −1 . Carbon to N uptake ratios averaged (±SD) 20.9 ± 11.7 (grams C to grams N) and were correlated with seston compositional ratios. Polysaccharide was the major end product of photosynthesis (as measured by 14 C incorporation) in measurements made at 100 μE∙m −2 ∙s −1 whereas protein was the primary end product during dark incubations. Samples enriched with NH 4 + and incubated at 100 μE∙m −2 ∙s −1 showed a significant increase in the 14 C incorporated into protein, at the expense of that incorporated into polysaccharide and low molecular weight metabolites. No differences in the pathway of C flow were observed in NH 4 + -enriched, dark-incubated samples. Nitrogen uptake estimates based on the percent of 14 C in the protein fraction were similar to those calculated by the 15 N technique." @default.
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- W2095175046 date "1984-10-01" @default.
- W2095175046 modified "2023-09-26" @default.
- W2095175046 title "Inorganic Nitrogen Uptake in Oligotrophic Lake Taupo, New Zealand" @default.
- W2095175046 doi "https://doi.org/10.1139/f84-177" @default.
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