FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095189121> ?p ?o ?g. }

• W2095189121 endingPage "96" @default.
• W2095189121 startingPage "89" @default.
• W2095189121 abstract "The increased interest in using fluorescein as a fluorescent probe in biology and medicine is associated with its distinct absorption and fluorescence signals that are transparent to biological samples. Herein, we characterize the binding mechanism of fluorescein inside human serum albumin which is used as a carrier and protector for fluorescein in medical applications. Binding of fluorescein in human serum albumin causes partial fluorescence quenching of the sole tryptophan residue in the protein (W214). The estimated W214-fluorescein distance (2.42 nm) and the calculated quenching rate constant ( k q = 5.13 × 10 12 M −1 s −1 ), both indicate binding of fluorescein in subdomain IIA. A site-competitive experiment shows that fluorescein is located in the warfarin binding pocket. The estimated binding constant ( K = 10,000 M −1 ) points to a moderate binding strength of the fluorescein–human serum albumin complex that should not affect the fluorescein release to the target when used as a probe. • Fluorescein bound to human serum albumin (HSA) is widely used in medical applications. • In this work, we investigated the binding mechanism of fluorescein with HSA. • We assigned a location for fluorescein inside HSA by performing a series of quenching experiments. • Fluorescein is found to be chemically stable inside HSA with moderate binding strength. • We found the local environment in the binding site to resemble that of water-DMSO mixture." @default.
• W2095189121 created "2016-06-24" @default.
• W2095189121 creator A5007888375 @default.
• W2095189121 creator A5056389437 @default.
• W2095189121 date "2014-11-01" @default.
• W2095189121 modified "2023-09-24" @default.
• W2095189121 title "Site-specific recognition of fluorescein by human serum albumin: A steady-state and time-resolved spectroscopic study" @default.
• W2095189121 cites W1506202992 @default.
• W2095189121 cites W1561686310 @default.
• W2095189121 cites W1965455689 @default.
• W2095189121 cites W1967667285 @default.
• W2095189121 cites W1973238993 @default.
• W2095189121 cites W1973387903 @default.
• W2095189121 cites W1976436661 @default.
• W2095189121 cites W1990898399 @default.
• W2095189121 cites W1991190990 @default.
• W2095189121 cites W1998122941 @default.
• W2095189121 cites W2010004274 @default.
• W2095189121 cites W2017674624 @default.
• W2095189121 cites W2020046442 @default.
• W2095189121 cites W2033162774 @default.
• W2095189121 cites W2055481883 @default.
• W2095189121 cites W2059164224 @default.
• W2095189121 cites W2060595131 @default.
• W2095189121 cites W2063393045 @default.
• W2095189121 cites W2068615039 @default.
• W2095189121 cites W2071055678 @default.
• W2095189121 cites W2085446806 @default.
• W2095189121 cites W2094174414 @default.
• W2095189121 cites W2094517427 @default.
• W2095189121 cites W2098358122 @default.
• W2095189121 cites W2105898915 @default.
• W2095189121 cites W2135059847 @default.
• W2095189121 cites W2143377531 @default.
• W2095189121 cites W2163350887 @default.
• W2095189121 cites W2163370265 @default.
• W2095189121 cites W2167373328 @default.
• W2095189121 cites W2314348380 @default.
• W2095189121 cites W2327356451 @default.
• W2095189121 cites W2334047574 @default.
• W2095189121 cites W2334371566 @default.
• W2095189121 cites W4253345025 @default.
• W2095189121 cites W4254187702 @default.
• W2095189121 doi "https://doi.org/10.1016/j.dyepig.2014.05.005" @default.
• W2095189121 hasPublicationYear "2014" @default.
• W2095189121 type Work @default.
• W2095189121 sameAs 2095189121 @default.
• W2095189121 citedByCount "26" @default.
• W2095189121 countsByYear W20951891212015 @default.
• W2095189121 countsByYear W20951891212016 @default.
• W2095189121 countsByYear W20951891212017 @default.
• W2095189121 countsByYear W20951891212018 @default.
• W2095189121 countsByYear W20951891212019 @default.
• W2095189121 countsByYear W20951891212020 @default.
• W2095189121 countsByYear W20951891212021 @default.
• W2095189121 countsByYear W20951891212022 @default.
• W2095189121 crossrefType "journal-article" @default.
• W2095189121 hasAuthorship W2095189121A5007888375 @default.
• W2095189121 hasAuthorship W2095189121A5056389437 @default.
• W2095189121 hasConcept C107824862 @default.
• W2095189121 hasConcept C120665830 @default.
• W2095189121 hasConcept C121332964 @default.
• W2095189121 hasConcept C121745418 @default.
• W2095189121 hasConcept C125287762 @default.
• W2095189121 hasConcept C12554922 @default.
• W2095189121 hasConcept C159985019 @default.
• W2095189121 hasConcept C165616093 @default.
• W2095189121 hasConcept C185592680 @default.
• W2095189121 hasConcept C192562407 @default.
• W2095189121 hasConcept C2776125364 @default.
• W2095189121 hasConcept C2777019933 @default.
• W2095189121 hasConcept C2778015030 @default.
• W2095189121 hasConcept C2778597219 @default.
• W2095189121 hasConcept C32786932 @default.
• W2095189121 hasConcept C43617362 @default.
• W2095189121 hasConcept C55493867 @default.
• W2095189121 hasConcept C86803240 @default.
• W2095189121 hasConcept C91881484 @default.
• W2095189121 hasConceptScore W2095189121C107824862 @default.
• W2095189121 hasConceptScore W2095189121C120665830 @default.
• W2095189121 hasConceptScore W2095189121C121332964 @default.
• W2095189121 hasConceptScore W2095189121C121745418 @default.
• W2095189121 hasConceptScore W2095189121C125287762 @default.
• W2095189121 hasConceptScore W2095189121C12554922 @default.
• W2095189121 hasConceptScore W2095189121C159985019 @default.
• W2095189121 hasConceptScore W2095189121C165616093 @default.
• W2095189121 hasConceptScore W2095189121C185592680 @default.
• W2095189121 hasConceptScore W2095189121C192562407 @default.
• W2095189121 hasConceptScore W2095189121C2776125364 @default.
• W2095189121 hasConceptScore W2095189121C2777019933 @default.
• W2095189121 hasConceptScore W2095189121C2778015030 @default.
• W2095189121 hasConceptScore W2095189121C2778597219 @default.
• W2095189121 hasConceptScore W2095189121C32786932 @default.
• W2095189121 hasConceptScore W2095189121C43617362 @default.
• W2095189121 hasConceptScore W2095189121C55493867 @default.
• W2095189121 hasConceptScore W2095189121C86803240 @default.
• W2095189121 hasConceptScore W2095189121C91881484 @default.
• W2095189121 hasLocation W20951891211 @default.

• next