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• W2095263399 abstract "Conditioned medium containing immune interferon (IFN) activity was prepared by stimulating spleen lymphocytes obtained from inbred SC chickens with 10 μg concanavalin A (Con A) for 48 h. Pretreatment of spleen cells with monoclonal antibody against CD4, but not CD8, abrogated IFN production suggesting that CD4+ lymphocytes are responsible for immune IFN production. Immune IFN was purified 25-fold from Con A conditioned medium using controlled-pore glass column chromatography resulting in an increase in specific antiviral activity from 7 to 3290 units mg−1. Partially purified immune IFN retained antiviral and macrophage-activating factor (MAF)-like activities. Normal peripheral blood macrophages, when cultured in the presence of partially purified immune IFN, showed a dose-dependent increase in cell surface major histocompatibility complex Class II antigen expression by flow cytometry. Northern blot analysis of mRNA obtained from IFN-treated macrophages showed a concomitant increase in Class II gene expression. This effect was more obvious in cells induced for 48 h than in those induced for 24 h. These results strongly suggest the existence of an avian homologue of the MAF-like activity." @default.
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• W2095263399 date "1994-12-01" @default.
• W2095263399 modified "2023-09-23" @default.
• W2095263399 title "Chicken interferon-mediated induction of major histocompatibility complex Class II antigens on peripheral blood monocytes" @default.
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• W2095263399 doi "https://doi.org/10.1016/0165-2427(94)90170-8" @default.
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