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- W2095285056 abstract "1. A modification of a previously published method for the disruption of the T-tubules of frog skeletal muscle is described. The modification permits the disruption of the T-tubules without the decline in resting potentials which was reported previously.2. The method for the disruption of the T-tubules involves the washout of glycerol following loading in a 400 mM glycerol Ringer solution. The modification consists of elevating the concentration of divalent cations in the Ringer used for glycerol washout.3. The optimum concentrations are 5 mM-Ca(2+) and 5 mM-Mg(2+) added as their chloride salts. Neither 10 mM-Ca(2+) nor 10 mM-Mg(2+) are as effective as the combination of each at 5 mM. Other concentrations gave less satisfactory results.4. The use of the modified technique provides a preparation which maintains 85-90 mV resting potentials for up to 6 or 8 hr but which will not contract in response to membrane depolarization." @default.
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- W2095285056 date "1971-05-01" @default.
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- W2095285056 title "The maintenance of resting potentials in glycerol-treated muscle fibres" @default.
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- W2095285056 doi "https://doi.org/10.1113/jphysiol.1971.sp009459" @default.
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