FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095309980> ?p ?o ?g. }

• W2095309980 endingPage "4550" @default.
• W2095309980 startingPage "4540" @default.
• W2095309980 abstract "Protein composition of cardiac sarcolemmal membranes was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Membranes were observed to contain about 20 polypeptide bands ranging from 18000 to 200 000 dalton mass. Out of these, six bands were prominent and together comprised 57% of the membrane protein. When sarcolemmal membranes, phosphorylated by [gamma-(32)P] ATP in the presence of Ca(2+) or Na+ with and without K+, were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at pH 2.4, the band III region (Mr 105 000) of gels was found to contain active sites of monomeric Ca-ATPase and (Na,K)ATPase. Bands I (Mr greater than 200 000), II (Mr 150 000), III (Mr 105 000), and VI (Mr 47 000) were accesible to trypsin; the extent of proteolysis was dependent on the time of exposure to, and the concentration of, trypsin (i.e, ratio of sarcolemmal protein/trypsin). Addition of molar sucrose protected sarcolemmal proteins from the tryptic proteolysis. Calcium transport was reduced by the action of trypsin; the degree of reduction was influenced by the time of exposure of membranes to trypsin as well as the concentration of trypsin. (Mg,Ca)ATPase activity, on the other hand, was elevated moderately at lower concentration and reduced at higher concentration of trypsin. Treatment with phospholipase C cium transport and (Mg,Ca)ATPase activity; electrophoretic patterns were unaffected by this treatment. Addition of lecithin to phospholipase C treated membranes produced a moderate increase in calcium transport. Exposure to Triton X-100 (1%) specifically solubilized three protein bands (Mr90 000, 67 000, and 57 000), whereas exposure to deoxycholate (1%) preferentially solubilized high-molecular-weight proteins, including band III (Mr 105 000); Lubrol-PX (1%) caused nonspecific solubilization of proteins, although the extent of solubilization with Lubrol-PX was considerably less than with either Triton or deoxycholate." @default.
• W2095309980 created "2016-06-24" @default.
• W2095309980 creator A5044801180 @default.
• W2095309980 creator A5090004135 @default.
• W2095309980 date "1978-10-17" @default.
• W2095309980 modified "2023-09-24" @default.
• W2095309980 title "Protein analysis of cardiac sarcolemma: effects of membrane-perturbing agents on membrane proteins and calcium transport" @default.
• W2095309980 cites W1484784503 @default.
• W2095309980 cites W1489291171 @default.
• W2095309980 cites W1497278434 @default.
• W2095309980 cites W1520266592 @default.
• W2095309980 cites W1523220410 @default.
• W2095309980 cites W1533496253 @default.
• W2095309980 cites W1542542098 @default.
• W2095309980 cites W1550580103 @default.
• W2095309980 cites W1558592232 @default.
• W2095309980 cites W1572315545 @default.
• W2095309980 cites W1583133549 @default.
• W2095309980 cites W1590300599 @default.
• W2095309980 cites W1595204022 @default.
• W2095309980 cites W1775749144 @default.
• W2095309980 cites W1971519888 @default.
• W2095309980 cites W1977799984 @default.
• W2095309980 cites W1980142140 @default.
• W2095309980 cites W1984838283 @default.
• W2095309980 cites W1985716109 @default.
• W2095309980 cites W1987550340 @default.
• W2095309980 cites W2002885745 @default.
• W2095309980 cites W2003035041 @default.
• W2095309980 cites W2003889958 @default.
• W2095309980 cites W2013882419 @default.
• W2095309980 cites W2017798009 @default.
• W2095309980 cites W2042843480 @default.
• W2095309980 cites W2063821350 @default.
• W2095309980 cites W2065637839 @default.
• W2095309980 cites W2073621425 @default.
• W2095309980 cites W2073879684 @default.
• W2095309980 cites W2088511380 @default.
• W2095309980 cites W2092513986 @default.
• W2095309980 cites W2105745018 @default.
• W2095309980 cites W2144763990 @default.
• W2095309980 cites W2163221467 @default.
• W2095309980 cites W2172587676 @default.
• W2095309980 cites W2247073492 @default.
• W2095309980 cites W2341577097 @default.
• W2095309980 cites W2414667409 @default.
• W2095309980 cites W4251600401 @default.
• W2095309980 cites W948655247 @default.
• W2095309980 doi "https://doi.org/10.1021/bi00614a028" @default.
• W2095309980 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/214104" @default.
• W2095309980 hasPublicationYear "1978" @default.
• W2095309980 type Work @default.
• W2095309980 sameAs 2095309980 @default.
• W2095309980 citedByCount "21" @default.
• W2095309980 crossrefType "journal-article" @default.
• W2095309980 hasAuthorship W2095309980A5044801180 @default.
• W2095309980 hasAuthorship W2095309980A5090004135 @default.
• W2095309980 hasConcept C116084860 @default.
• W2095309980 hasConcept C178790620 @default.
• W2095309980 hasConcept C181199279 @default.
• W2095309980 hasConcept C185592680 @default.
• W2095309980 hasConcept C199096232 @default.
• W2095309980 hasConcept C23265538 @default.
• W2095309980 hasConcept C2778597717 @default.
• W2095309980 hasConcept C2778913249 @default.
• W2095309980 hasConcept C2780340462 @default.
• W2095309980 hasConcept C41625074 @default.
• W2095309980 hasConcept C43617362 @default.
• W2095309980 hasConcept C519063684 @default.
• W2095309980 hasConcept C537181965 @default.
• W2095309980 hasConcept C55493867 @default.
• W2095309980 hasConceptScore W2095309980C116084860 @default.
• W2095309980 hasConceptScore W2095309980C178790620 @default.
• W2095309980 hasConceptScore W2095309980C181199279 @default.
• W2095309980 hasConceptScore W2095309980C185592680 @default.
• W2095309980 hasConceptScore W2095309980C199096232 @default.
• W2095309980 hasConceptScore W2095309980C23265538 @default.
• W2095309980 hasConceptScore W2095309980C2778597717 @default.
• W2095309980 hasConceptScore W2095309980C2778913249 @default.
• W2095309980 hasConceptScore W2095309980C2780340462 @default.
• W2095309980 hasConceptScore W2095309980C41625074 @default.
• W2095309980 hasConceptScore W2095309980C43617362 @default.
• W2095309980 hasConceptScore W2095309980C519063684 @default.
• W2095309980 hasConceptScore W2095309980C537181965 @default.
• W2095309980 hasConceptScore W2095309980C55493867 @default.
• W2095309980 hasIssue "21" @default.
• W2095309980 hasLocation W20953099801 @default.
• W2095309980 hasLocation W20953099802 @default.
• W2095309980 hasOpenAccess W2095309980 @default.
• W2095309980 hasPrimaryLocation W20953099801 @default.
• W2095309980 hasRelatedWork W1536513137 @default.
• W2095309980 hasRelatedWork W1985429117 @default.
• W2095309980 hasRelatedWork W1991618972 @default.
• W2095309980 hasRelatedWork W2004890815 @default.
• W2095309980 hasRelatedWork W2021037556 @default.
• W2095309980 hasRelatedWork W2024094716 @default.
• W2095309980 hasRelatedWork W2078745256 @default.
• W2095309980 hasRelatedWork W2095309980 @default.

• next