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- W2095330168 abstract "Abstract The assumption that the amount of amylase secreted by parotid glands is dependent on the glandular content is often made but seldom substantiated. This association was tested in vitro in parotid tissue. Fasting, feeding and feeding after pre-ganglionic cervical sympathectomy provided contrasting levels of glandular amylase. The subsequent release into fortified Krebs' medium was measured. Secretion was induced by 4 × 10 −7 M carbachol or isoprenaline. Only when the combined results from fasted and fed animals or fed sympathectomized and fed control rats were analysed were the total amounts of amylase released into the medium positively related to the initial amounts in the glands. This applied to spontaneously secreting, carbachol- and isoprenaline-stimulated tissue. When the results from each group were considered separately, this relationship was absent. When the secretion of amylase was expressed as a percentage of the total amylase in the gland, less was released from repleted glands, particularly in those stimulated by isoprenaline. Pre-treatment of tissues in vitro for 4 h with 1.25 × 10 −4 M colchicine impaired amylase release in response to isoprenaline only. Possible explanations for these findings relate to the different distribution and rate of synthesis of amylase in acinar cells of fasted and fed rats." @default.
- W2095330168 created "2016-06-24" @default.
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- W2095330168 date "1981-01-01" @default.
- W2095330168 modified "2023-09-25" @default.
- W2095330168 title "The relation between amylase secreted in vitro by rat parotid tissue and the amylase concentration in the glands" @default.
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- W2095330168 doi "https://doi.org/10.1016/0003-9969(81)90153-9" @default.
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