Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095384414> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2095384414 abstract "Abstract Background Quantitative evaluation of mosaics of photoreceptors and neurons is essential in studies on development, aging and degeneration of the retina. Manual counting of samples is a time consuming procedure while attempts to automatization are subject to various restrictions from biological and preparation variability leading to both over- and underestimation of cell numbers. Here we present an adaptive algorithm to overcome many of these problems. Digital micrographs were obtained from cone photoreceptor mosaics visualized by anti-opsin immuno-cytochemistry in retinal wholemounts from a variety of mammalian species including primates. Segmentation of photoreceptors (from background, debris, blood vessels, other cell types) was performed by a procedure based on Rudin-Osher-Fatemi total variation (TV) denoising. Once 3 parameters are manually adjusted based on a sample, similarly structured images can be batch processed. The module is implemented in MATLAB and fully documented online. Results The object recognition procedure was tested on samples with a typical range of signal and background variations. We obtained results with error ratios of less than 10% in 16 of 18 samples and a mean error of less than 6% compared to manual counts. Conclusions The presented method provides a traceable module for automated acquisition of retinal cell density data. Remaining errors, including addition of background items, splitting or merging of objects might be further reduced by introduction of additional parameters. The module may be integrated into extended environments with features such as 3D-acquisition and recognition." @default.
- W2095384414 created "2016-06-24" @default.
- W2095384414 creator A5017147366 @default.
- W2095384414 creator A5031177851 @default.
- W2095384414 creator A5048637561 @default.
- W2095384414 creator A5060778033 @default.
- W2095384414 date "2013-10-20" @default.
- W2095384414 modified "2023-09-27" @default.
- W2095384414 title "Computer-assisted counting of retinal cells by automatic segmentation after TV denoising" @default.
- W2095384414 cites W1579559187 @default.
- W2095384414 cites W1994098742 @default.
- W2095384414 cites W2000594266 @default.
- W2095384414 cites W2014928828 @default.
- W2095384414 cites W2039764427 @default.
- W2095384414 cites W2056855078 @default.
- W2095384414 cites W2069614805 @default.
- W2095384414 cites W2074291264 @default.
- W2095384414 cites W2080237929 @default.
- W2095384414 cites W2081508787 @default.
- W2095384414 cites W2089792655 @default.
- W2095384414 cites W2091342368 @default.
- W2095384414 cites W2092663520 @default.
- W2095384414 cites W2103559027 @default.
- W2095384414 cites W2107554012 @default.
- W2095384414 cites W2116425999 @default.
- W2095384414 cites W2119680805 @default.
- W2095384414 cites W2123871098 @default.
- W2095384414 cites W2126978393 @default.
- W2095384414 cites W2127731890 @default.
- W2095384414 cites W40037269 @default.
- W2095384414 doi "https://doi.org/10.1186/1471-2415-13-59" @default.
- W2095384414 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3835550" @default.
- W2095384414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24138794" @default.
- W2095384414 hasPublicationYear "2013" @default.
- W2095384414 type Work @default.
- W2095384414 sameAs 2095384414 @default.
- W2095384414 citedByCount "3" @default.
- W2095384414 countsByYear W20953844142015 @default.
- W2095384414 countsByYear W20953844142017 @default.
- W2095384414 countsByYear W20953844142019 @default.
- W2095384414 crossrefType "journal-article" @default.
- W2095384414 hasAuthorship W2095384414A5017147366 @default.
- W2095384414 hasAuthorship W2095384414A5031177851 @default.
- W2095384414 hasAuthorship W2095384414A5048637561 @default.
- W2095384414 hasAuthorship W2095384414A5060778033 @default.
- W2095384414 hasBestOaLocation W20953844141 @default.
- W2095384414 hasConcept C115961682 @default.
- W2095384414 hasConcept C153180895 @default.
- W2095384414 hasConcept C154945302 @default.
- W2095384414 hasConcept C169760540 @default.
- W2095384414 hasConcept C2777093970 @default.
- W2095384414 hasConcept C31972630 @default.
- W2095384414 hasConcept C41008148 @default.
- W2095384414 hasConcept C71924100 @default.
- W2095384414 hasConcept C86803240 @default.
- W2095384414 hasConcept C89600930 @default.
- W2095384414 hasConcept C99498987 @default.
- W2095384414 hasConceptScore W2095384414C115961682 @default.
- W2095384414 hasConceptScore W2095384414C153180895 @default.
- W2095384414 hasConceptScore W2095384414C154945302 @default.
- W2095384414 hasConceptScore W2095384414C169760540 @default.
- W2095384414 hasConceptScore W2095384414C2777093970 @default.
- W2095384414 hasConceptScore W2095384414C31972630 @default.
- W2095384414 hasConceptScore W2095384414C41008148 @default.
- W2095384414 hasConceptScore W2095384414C71924100 @default.
- W2095384414 hasConceptScore W2095384414C86803240 @default.
- W2095384414 hasConceptScore W2095384414C89600930 @default.
- W2095384414 hasConceptScore W2095384414C99498987 @default.
- W2095384414 hasIssue "1" @default.
- W2095384414 hasLocation W20953844141 @default.
- W2095384414 hasLocation W20953844142 @default.
- W2095384414 hasLocation W20953844143 @default.
- W2095384414 hasLocation W20953844144 @default.
- W2095384414 hasOpenAccess W2095384414 @default.
- W2095384414 hasPrimaryLocation W20953844141 @default.
- W2095384414 hasRelatedWork W1669643531 @default.
- W2095384414 hasRelatedWork W2005437358 @default.
- W2095384414 hasRelatedWork W2008656436 @default.
- W2095384414 hasRelatedWork W2023558673 @default.
- W2095384414 hasRelatedWork W2039154422 @default.
- W2095384414 hasRelatedWork W2110230079 @default.
- W2095384414 hasRelatedWork W2122581818 @default.
- W2095384414 hasRelatedWork W2134924024 @default.
- W2095384414 hasRelatedWork W2517104666 @default.
- W2095384414 hasRelatedWork W2182382398 @default.
- W2095384414 hasVolume "13" @default.
- W2095384414 isParatext "false" @default.
- W2095384414 isRetracted "false" @default.
- W2095384414 magId "2095384414" @default.
- W2095384414 workType "article" @default.