FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095397678> ?p ?o ?g. }

• W2095397678 abstract "The transcriptional activity of various lengths of the 5'-untranslated region (UTR) of the murine LH receptor (R) gene were studied using the luciferase reporter gene in transiently transfected mouse Leydig tumor cells (mLTC-1). Chinese hamster ovarian (CHO) and HeLa cells were used as controls. The basal transcriptional promoter activity in mLTC-1 cells resided within the first 173 base pairs (bp) of the 5'-UTR. Placing an LHR promoter fragment (bases -715/ -56) in front of the Herpes simplex virus thymidine kinase (TK) minimal promoter resulted in a 7-fold increase in luciferase activity. Deletion of bases -56 to -173 of the above construct totally abolished the increased luciferase activity, brought about by the LHR promoter sequences. Basically similar results on LHR promoter function were observed using CHO cells. In contrast, no LHR promoter activity was detected in HeLa cells, indicating a cell specific nature of its function. The first 173 bp promoter domain is GC-rich, with several SP-1 binding domains, and it bound specifically nuclear proteins isolated from mLTC-1 and HeLa cells. RNAse protection assays reconfirmed the presence of several transcription initiation sites within the first 310 bp of the 5'-UTR, also in the absence of the cognate LHR coding sequences. The most distal site at bp -310 did not function in the absence of the first 173 bp of the 5'-UTR. Other transcription initiation sites were identified closer to the translation initiation site. hCG (50 micrograms/l), 8-bromo (Br)-cAMP (100 mumol/l) and cholera toxin (100 microgram/l) displayed qualitatively similar negative effects on the LHR promoter activity in the transfected mLTC-1 cells when the constructs containing at least the first 565 bp of the LHR 5'-UTR were used, but the inhibitory effects were greatly decreased in constructs containing < or = 304 bp of the promoter region. Hence, the hCG/cAMP associated inhibitory effects interact with region(s) located mainly between bp -565 and -305 of the LHR promoter. The inhibitory role of cAMP on LHR gene expression was also confirmed at the level of hCO-binding and hCG stimulated cAMP production of mLTC-1 cells. In conclusion, the current results elucidate the cis- and trans-acting elements in the regulation of expression of the murine LHR gene in cultured mouse Leydig cells. The minimal basal promoter activity is within the first 173 nucleotides of the 5'-UTR and the structural elements of the negative LHR regulation by the cognate hormone and elevated cAMP levels are mainly located within nucleotides -305 to -565 of the 5'-UTR. The function of the murine LHR promoter is similar to, though not identical with that of the rat, but at variance with that of the human LHR gene." @default.
• W2095397678 created "2016-06-24" @default.
• W2095397678 creator A5000385705 @default.
• W2095397678 creator A5018252046 @default.
• W2095397678 creator A5059776672 @default.
• W2095397678 creator A5088392007 @default.
• W2095397678 creator A5091508460 @default.
• W2095397678 date "1996-05-01" @default.
• W2095397678 modified "2023-09-27" @default.
• W2095397678 title "Regulation of function of the murine luteinizing hormone receptor promoter by cis- and trans-acting elements in mouse Leydig tumor cells" @default.
• W2095397678 cites W1481096871 @default.
• W2095397678 cites W1485550032 @default.
• W2095397678 cites W1499455100 @default.
• W2095397678 cites W1526692496 @default.
• W2095397678 cites W1550494424 @default.
• W2095397678 cites W1566427805 @default.
• W2095397678 cites W1586241513 @default.
• W2095397678 cites W1591828201 @default.
• W2095397678 cites W1982598173 @default.
• W2095397678 cites W1982684652 @default.
• W2095397678 cites W1982977098 @default.
• W2095397678 cites W1990989603 @default.
• W2095397678 cites W1993243592 @default.
• W2095397678 cites W2006466412 @default.
• W2095397678 cites W2017382801 @default.
• W2095397678 cites W2018889720 @default.
• W2095397678 cites W2028021946 @default.
• W2095397678 cites W2031206832 @default.
• W2095397678 cites W2034325295 @default.
• W2095397678 cites W2042585970 @default.
• W2095397678 cites W2064360396 @default.
• W2095397678 cites W2084519984 @default.
• W2095397678 cites W2088339225 @default.
• W2095397678 cites W2102225311 @default.
• W2095397678 cites W2166180405 @default.
• W2095397678 cites W4238051583 @default.
• W2095397678 cites W4240919184 @default.
• W2095397678 cites W4244697114 @default.
• W2095397678 cites W4248029870 @default.
• W2095397678 cites W4250358723 @default.
• W2095397678 cites W4250789773 @default.
• W2095397678 cites W4251291546 @default.
• W2095397678 cites W4253758070 @default.
• W2095397678 cites W4255363694 @default.
• W2095397678 cites W4255515260 @default.
• W2095397678 doi "https://doi.org/10.1016/0303-7207(96)03815-4" @default.
• W2095397678 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8807640" @default.
• W2095397678 hasPublicationYear "1996" @default.
• W2095397678 type Work @default.
• W2095397678 sameAs 2095397678 @default.
• W2095397678 citedByCount "23" @default.
• W2095397678 countsByYear W20953976782019 @default.
• W2095397678 crossrefType "journal-article" @default.
• W2095397678 hasAuthorship W2095397678A5000385705 @default.
• W2095397678 hasAuthorship W2095397678A5018252046 @default.
• W2095397678 hasAuthorship W2095397678A5059776672 @default.
• W2095397678 hasAuthorship W2095397678A5088392007 @default.
• W2095397678 hasAuthorship W2095397678A5091508460 @default.
• W2095397678 hasConcept C101762097 @default.
• W2095397678 hasConcept C104317684 @default.
• W2095397678 hasConcept C105580179 @default.
• W2095397678 hasConcept C111335760 @default.
• W2095397678 hasConcept C138885662 @default.
• W2095397678 hasConcept C150194340 @default.
• W2095397678 hasConcept C153911025 @default.
• W2095397678 hasConcept C170493617 @default.
• W2095397678 hasConcept C175656101 @default.
• W2095397678 hasConcept C179926584 @default.
• W2095397678 hasConcept C2777366897 @default.
• W2095397678 hasConcept C41895202 @default.
• W2095397678 hasConcept C54009773 @default.
• W2095397678 hasConcept C54355233 @default.
• W2095397678 hasConcept C81885089 @default.
• W2095397678 hasConcept C86803240 @default.
• W2095397678 hasConcept C89604277 @default.
• W2095397678 hasConceptScore W2095397678C101762097 @default.
• W2095397678 hasConceptScore W2095397678C104317684 @default.
• W2095397678 hasConceptScore W2095397678C105580179 @default.
• W2095397678 hasConceptScore W2095397678C111335760 @default.
• W2095397678 hasConceptScore W2095397678C138885662 @default.
• W2095397678 hasConceptScore W2095397678C150194340 @default.
• W2095397678 hasConceptScore W2095397678C153911025 @default.
• W2095397678 hasConceptScore W2095397678C170493617 @default.
• W2095397678 hasConceptScore W2095397678C175656101 @default.
• W2095397678 hasConceptScore W2095397678C179926584 @default.
• W2095397678 hasConceptScore W2095397678C2777366897 @default.
• W2095397678 hasConceptScore W2095397678C41895202 @default.
• W2095397678 hasConceptScore W2095397678C54009773 @default.
• W2095397678 hasConceptScore W2095397678C54355233 @default.
• W2095397678 hasConceptScore W2095397678C81885089 @default.
• W2095397678 hasConceptScore W2095397678C86803240 @default.
• W2095397678 hasConceptScore W2095397678C89604277 @default.
• W2095397678 hasLocation W20953976781 @default.
• W2095397678 hasLocation W20953976782 @default.
• W2095397678 hasOpenAccess W2095397678 @default.
• W2095397678 hasPrimaryLocation W20953976781 @default.
• W2095397678 hasRelatedWork W2043847594 @default.
• W2095397678 hasRelatedWork W2052266897 @default.
• W2095397678 hasRelatedWork W2077063678 @default.
• W2095397678 hasRelatedWork W2156706857 @default.

• next