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• W2095410114 abstract "Abstract The effect of a long‐term adaptation of rainbow trout to 8 and 18°C combined with a corn oil‐or a fish oil‐supplemented diet on the characteristics of the spermatozoan plasma membrane was investigated. The experiment lasted up to 22 mon during which spermatozoa were collected from the mature males. Spermatozoan plasma membranes were isolated by nitrogen cavitation, and the cholesterol content, phospholipid composition and fatty acid pattern were investigated. Membrane viscosity was assessed on whole cells by electron spin resonance using spin‐labeled phospholipids. Neither diet nor rearing temperature influenced the cholesterol content of the plasma membrane nor the phospholipid class distribution. The rearing temperature of the broodstock only slightly affected the phospholipid fatty acids. A minor decrease in 18∶0 and increase in monounsaturated fatty acids was observed for the cold‐adapted fish. These modifications were not sufficient to affect membrane fluidity, and we conclude that trout spermatozoa do not display any homeoviscous adaptations in these conditions. On the contrary, the dietary fatty acid intake greatly modified the fatty acid profile of plasma membrane phospholipids. The fish oil‐fed trout displayed a much higher n−3/n−6 fatty acid ratio than did the corn oil‐fed ones, but the 22∶6n−3 levels remained unchanged. Modifications in plasma membrane composition by the diet were obtained although neither of the two diets was deficient in essential fatty acids. The enrichment in n−3 fatty acids, however, did not affect plasma membrane fluidity which was unchanged by the diets." @default.
• W2095410114 created "2016-06-24" @default.
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• W2095410114 date "1995-01-01" @default.
• W2095410114 modified "2023-09-29" @default.
• W2095410114 title "Thermal acclimation and dietary lipids alter the composition, but not fluidity, of trout sperm plasma membrane" @default.
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• W2095410114 doi "https://doi.org/10.1007/bf02537038" @default.
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